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Effects of Epigallocatechin Gallate on Tert-Butyl Hydroperoxide-Induced Mitochondrial Dysfunction in Rat Liver Mitochondria and Hepatocytes
Joint Authors
Kučera, Otto
Endlicher, René
Červinková, Zuzana
Mezera, Vojtech
Sobotka, Ondrej
Drahota, Zdenek
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-12-15
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Epigallocatechin gallate (EGCG) is a green tea antioxidant with adverse effects on rat liver mitochondria and hepatocytes at high doses.
Here, we assessed whether low doses of EGCG would protect these systems from damage induced by tert-butyl hydroperoxide (tBHP).
Rat liver mitochondria or permeabilized rat hepatocytes were pretreated with EGCG and then exposed to tBHP.
Oxygen consumption, mitochondrial membrane potential (MMP), and mitochondrial retention capacity for calcium were measured.
First, 50 μM EGCG or 0.25 mM tBHP alone increased State 4 Complex I-driven respiration, thus demonstrating uncoupling effects; tBHP also inhibited State 3 ADP-stimulated respiration.
Then, the coexposure to 0.25 mM tBHP and 50 μM EGCG induced a trend of further decline in the respiratory control ratio beyond that observed upon tBHP exposure alone.
EGCG had no effect on MMP and no effect, in concentrations up to 50 μM, on mitochondrial calcium retention capacity.
tBHP led to a decline in both MMP and mitochondrial retention capacity for calcium; these effects were not changed by pretreatment with EGCG.
In addition, EGCG dose-dependently enhanced hydrogen peroxide formation in a cell- and mitochondria-free medium.
Conclusion.
Moderate nontoxic doses of EGCG were not able to protect rat liver mitochondria and hepatocytes from tBHP-induced mitochondrial dysfunction.
American Psychological Association (APA)
Mezera, Vojtech& Endlicher, René& Kučera, Otto& Sobotka, Ondrej& Drahota, Zdenek& Červinková, Zuzana. 2016. Effects of Epigallocatechin Gallate on Tert-Butyl Hydroperoxide-Induced Mitochondrial Dysfunction in Rat Liver Mitochondria and Hepatocytes. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1114215
Modern Language Association (MLA)
Mezera, Vojtech…[et al.]. Effects of Epigallocatechin Gallate on Tert-Butyl Hydroperoxide-Induced Mitochondrial Dysfunction in Rat Liver Mitochondria and Hepatocytes. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1114215
American Medical Association (AMA)
Mezera, Vojtech& Endlicher, René& Kučera, Otto& Sobotka, Ondrej& Drahota, Zdenek& Červinková, Zuzana. Effects of Epigallocatechin Gallate on Tert-Butyl Hydroperoxide-Induced Mitochondrial Dysfunction in Rat Liver Mitochondria and Hepatocytes. Oxidative Medicine and Cellular Longevity. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1114215
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1114215