Nuclear Factor (Erythroid-Derived)-Related Factor 2-Associated Retinal Pigment Epithelial Cell Protection under Blue Light-Induced Oxidative Stress
المؤلفون المشاركون
Takayama, Kei
Kaneko, Hiroki
Kataoka, Keiko
Kimoto, Reona
Hwang, Shiang-Jyi
Ye, Fuxiang
Nagasaka, Yosuke
Tsunekawa, Taichi
Matsuura, Toshiyuki
Nonobe, Norie
Ito, Yasuki
Terasaki, Hiroko
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-09-27
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Purpose.
It is a matter of increasing concern that exposure to light-emitting diodes (LED), particularly blue light (BL), damages retinal cells.
This study aimed to investigate the retinal pigment epithelium (RPE) damage caused by BL and to elucidate the role of nuclear factor (erythroid-derived)-related factor 2 (Nrf2) in the pathogenesis of BL-induced RPE damage.
Methods.
ARPE-19, a human RPE cell line, and mouse primary RPE cells from wild-type and Nrf2 knockout (Nrf2 −/−) mice were cultured under blue LED exposure (intermediate wavelength, 450 nm).
Cell death rate and reactive oxygen species (ROS) generation were measured.
TUNEL staining was performed to detect apoptosis.
Real-time polymerase chain reaction was performed on NRF2 mRNA, and western blotting was performed to detect Nrf2 proteins in the nucleus or cytoplasm of RPE cells.
Results.
BL exposure increased cell death rate and ROS generation in ARPE-19 cells in a time-dependent manner; cell death was caused by apoptosis.
Moreover, BL exposure induced NRF2 mRNA upregulation and Nrf2 nuclear translocation in RPE.
Cell death rate was significantly higher in RPE cells from Nrf2 −/− mice than from wild-type mice.
Conclusions.
The Nrf2 pathway plays an important role in protecting RPE cells against BL-induced oxidative stress.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Takayama, Kei& Kaneko, Hiroki& Kataoka, Keiko& Kimoto, Reona& Hwang, Shiang-Jyi& Ye, Fuxiang…[et al.]. 2016. Nuclear Factor (Erythroid-Derived)-Related Factor 2-Associated Retinal Pigment Epithelial Cell Protection under Blue Light-Induced Oxidative Stress. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1114426
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Takayama, Kei…[et al.]. Nuclear Factor (Erythroid-Derived)-Related Factor 2-Associated Retinal Pigment Epithelial Cell Protection under Blue Light-Induced Oxidative Stress. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1114426
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Takayama, Kei& Kaneko, Hiroki& Kataoka, Keiko& Kimoto, Reona& Hwang, Shiang-Jyi& Ye, Fuxiang…[et al.]. Nuclear Factor (Erythroid-Derived)-Related Factor 2-Associated Retinal Pigment Epithelial Cell Protection under Blue Light-Induced Oxidative Stress. Oxidative Medicine and Cellular Longevity. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1114426
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1114426
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر