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Nuclear Factor (Erythroid-Derived)-Related Factor 2-Associated Retinal Pigment Epithelial Cell Protection under Blue Light-Induced Oxidative Stress
Joint Authors
Takayama, Kei
Kaneko, Hiroki
Kataoka, Keiko
Kimoto, Reona
Hwang, Shiang-Jyi
Ye, Fuxiang
Nagasaka, Yosuke
Tsunekawa, Taichi
Matsuura, Toshiyuki
Nonobe, Norie
Ito, Yasuki
Terasaki, Hiroko
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-09-27
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Purpose.
It is a matter of increasing concern that exposure to light-emitting diodes (LED), particularly blue light (BL), damages retinal cells.
This study aimed to investigate the retinal pigment epithelium (RPE) damage caused by BL and to elucidate the role of nuclear factor (erythroid-derived)-related factor 2 (Nrf2) in the pathogenesis of BL-induced RPE damage.
Methods.
ARPE-19, a human RPE cell line, and mouse primary RPE cells from wild-type and Nrf2 knockout (Nrf2 −/−) mice were cultured under blue LED exposure (intermediate wavelength, 450 nm).
Cell death rate and reactive oxygen species (ROS) generation were measured.
TUNEL staining was performed to detect apoptosis.
Real-time polymerase chain reaction was performed on NRF2 mRNA, and western blotting was performed to detect Nrf2 proteins in the nucleus or cytoplasm of RPE cells.
Results.
BL exposure increased cell death rate and ROS generation in ARPE-19 cells in a time-dependent manner; cell death was caused by apoptosis.
Moreover, BL exposure induced NRF2 mRNA upregulation and Nrf2 nuclear translocation in RPE.
Cell death rate was significantly higher in RPE cells from Nrf2 −/− mice than from wild-type mice.
Conclusions.
The Nrf2 pathway plays an important role in protecting RPE cells against BL-induced oxidative stress.
American Psychological Association (APA)
Takayama, Kei& Kaneko, Hiroki& Kataoka, Keiko& Kimoto, Reona& Hwang, Shiang-Jyi& Ye, Fuxiang…[et al.]. 2016. Nuclear Factor (Erythroid-Derived)-Related Factor 2-Associated Retinal Pigment Epithelial Cell Protection under Blue Light-Induced Oxidative Stress. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1114426
Modern Language Association (MLA)
Takayama, Kei…[et al.]. Nuclear Factor (Erythroid-Derived)-Related Factor 2-Associated Retinal Pigment Epithelial Cell Protection under Blue Light-Induced Oxidative Stress. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1114426
American Medical Association (AMA)
Takayama, Kei& Kaneko, Hiroki& Kataoka, Keiko& Kimoto, Reona& Hwang, Shiang-Jyi& Ye, Fuxiang…[et al.]. Nuclear Factor (Erythroid-Derived)-Related Factor 2-Associated Retinal Pigment Epithelial Cell Protection under Blue Light-Induced Oxidative Stress. Oxidative Medicine and Cellular Longevity. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1114426
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1114426