CLOCK Promotes Endothelial Damage by Inducing Autophagy through Reactive Oxygen Species
المؤلفون المشاركون
Guo, Daqiao
Lin, Changpo
Shi, Zhenyu
Qian, Ruizhe
Liu, Jianjun
Fan, Longhua
Tang, Xiao
Li, Xu
Li, Xiaobo
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-12-12
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
A number of recent studies have implicated that autophagy was activated by reactive oxygen species (ROS).
Our previous report indicated that CLOCK increased the accumulation of ROS under hypoxic conditions.
In this study, we investigated the mechanisms by which CLOCK mediated endothelial damage, focusing on the involvement of oxidative damage and autophagy.
Overexpression of CLOCK in human umbilical vein endothelial cells (HUVECs) showed inhibition of cell proliferation and higher autophagosome with an increased expression of Beclin1 and LC3-I/II under hypoxic conditions.
In contrast, CLOCK silencing reversed these effects.
Interestingly, pretreatment with 3-methyladenine (3-MA) resulted in the attenuation of CLOCK-induced cell autophagy and but did not influence the production of intracellular reactive oxygen species (ROS).
Furthermore, Tiron (4,5-dihydroxy-1,3-benzene disulfonic acid-disodium salt), a ROS scavenger, significantly attenuated CLOCK-induced cell autophagy.
In addition, we found that overexpression of CLOCK had no significant effects on the production of ROS and expression of Beclin1 and LC3-I/II under normoxic conditions in HUVEC.
In this present investigation, our results suggested a novel mechanism of action of CLOCK in HUVECs, opening up the possibility of targeting CLOCK for the treatment of vascular diseases.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Tang, Xiao& Lin, Changpo& Guo, Daqiao& Qian, Ruizhe& Li, Xiaobo& Shi, Zhenyu…[et al.]. 2016. CLOCK Promotes Endothelial Damage by Inducing Autophagy through Reactive Oxygen Species. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1114789
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Tang, Xiao…[et al.]. CLOCK Promotes Endothelial Damage by Inducing Autophagy through Reactive Oxygen Species. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1114789
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Tang, Xiao& Lin, Changpo& Guo, Daqiao& Qian, Ruizhe& Li, Xiaobo& Shi, Zhenyu…[et al.]. CLOCK Promotes Endothelial Damage by Inducing Autophagy through Reactive Oxygen Species. Oxidative Medicine and Cellular Longevity. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1114789
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1114789
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر