Expression of CD24 in Human Bone Marrow-Derived Mesenchymal Stromal Cells Is Regulated by TGFβ3 and Induces a Myofibroblast-Like Genotype

المؤلفون المشاركون

Buettner, Manuela
Neunaber, C.
Schäck, Luisa Marilena
Wirth, Alexander
Hoffmann, Andrea
Noack, Sandra
Krettek, Christian

المصدر

Stem Cells International

العدد

المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-13، 13ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2015-12-14

دولة النشر

مصر

عدد الصفحات

13

الملخص EN

Human bone marrow-derived stromal cells (hBMSCs) derived from the adult organism hold great promise for diverse settings in regenerative medicine.

Therefore a more complete understanding of hBMSC biology to fully exploit the cells’ potential for clinical settings is important.

The protein CD24 has been reported to be involved in a diverse range of processes such as cancer, adaptive immunity, inflammation, and autoimmune diseases in other cell types.

Its expression in hBMSCs, which has not yet been analyzed, may add an important aspect in the understanding of hBMSC biology.

The present study therefore analyzes the expression, regulation, and functional implication of the surface protein CD24 in hBMSCs.

Methods used are stimulation studies with TGF beta as well as shRNA-mediated knockdown and overexpression of CD24 followed by microarray, immunocytochemistry, and flow cytometric analyses.

To our knowledge, we demonstrate for the first time that the expression of CD24 is an inherent property of hBMSCs.

Importantly, the data links the upregulation of CD24 to the adoption of a myofibroblast-like gene expression pattern in hBMSCs.

We demonstrate that CD24 is an important modulator in transforming growth factor beta 3 (TGFβ3) signaling with a reciprocal regulatory relationship between these two proteins.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Schäck, Luisa Marilena& Buettner, Manuela& Wirth, Alexander& Neunaber, C.& Krettek, Christian& Hoffmann, Andrea…[et al.]. 2015. Expression of CD24 in Human Bone Marrow-Derived Mesenchymal Stromal Cells Is Regulated by TGFβ3 and Induces a Myofibroblast-Like Genotype. Stem Cells International،Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1116004

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Schäck, Luisa Marilena…[et al.]. Expression of CD24 in Human Bone Marrow-Derived Mesenchymal Stromal Cells Is Regulated by TGFβ3 and Induces a Myofibroblast-Like Genotype. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-13.
https://search.emarefa.net/detail/BIM-1116004

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Schäck, Luisa Marilena& Buettner, Manuela& Wirth, Alexander& Neunaber, C.& Krettek, Christian& Hoffmann, Andrea…[et al.]. Expression of CD24 in Human Bone Marrow-Derived Mesenchymal Stromal Cells Is Regulated by TGFβ3 and Induces a Myofibroblast-Like Genotype. Stem Cells International. 2015. Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1116004

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1116004