Expression of CD24 in Human Bone Marrow-Derived Mesenchymal Stromal Cells Is Regulated by TGFβ3 and Induces a Myofibroblast-Like Genotype
Joint Authors
Buettner, Manuela
Neunaber, C.
Schäck, Luisa Marilena
Wirth, Alexander
Hoffmann, Andrea
Noack, Sandra
Krettek, Christian
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-12-14
Country of Publication
Egypt
No. of Pages
13
Abstract EN
Human bone marrow-derived stromal cells (hBMSCs) derived from the adult organism hold great promise for diverse settings in regenerative medicine.
Therefore a more complete understanding of hBMSC biology to fully exploit the cells’ potential for clinical settings is important.
The protein CD24 has been reported to be involved in a diverse range of processes such as cancer, adaptive immunity, inflammation, and autoimmune diseases in other cell types.
Its expression in hBMSCs, which has not yet been analyzed, may add an important aspect in the understanding of hBMSC biology.
The present study therefore analyzes the expression, regulation, and functional implication of the surface protein CD24 in hBMSCs.
Methods used are stimulation studies with TGF beta as well as shRNA-mediated knockdown and overexpression of CD24 followed by microarray, immunocytochemistry, and flow cytometric analyses.
To our knowledge, we demonstrate for the first time that the expression of CD24 is an inherent property of hBMSCs.
Importantly, the data links the upregulation of CD24 to the adoption of a myofibroblast-like gene expression pattern in hBMSCs.
We demonstrate that CD24 is an important modulator in transforming growth factor beta 3 (TGFβ3) signaling with a reciprocal regulatory relationship between these two proteins.
American Psychological Association (APA)
Schäck, Luisa Marilena& Buettner, Manuela& Wirth, Alexander& Neunaber, C.& Krettek, Christian& Hoffmann, Andrea…[et al.]. 2015. Expression of CD24 in Human Bone Marrow-Derived Mesenchymal Stromal Cells Is Regulated by TGFβ3 and Induces a Myofibroblast-Like Genotype. Stem Cells International،Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1116004
Modern Language Association (MLA)
Schäck, Luisa Marilena…[et al.]. Expression of CD24 in Human Bone Marrow-Derived Mesenchymal Stromal Cells Is Regulated by TGFβ3 and Induces a Myofibroblast-Like Genotype. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-13.
https://search.emarefa.net/detail/BIM-1116004
American Medical Association (AMA)
Schäck, Luisa Marilena& Buettner, Manuela& Wirth, Alexander& Neunaber, C.& Krettek, Christian& Hoffmann, Andrea…[et al.]. Expression of CD24 in Human Bone Marrow-Derived Mesenchymal Stromal Cells Is Regulated by TGFβ3 and Induces a Myofibroblast-Like Genotype. Stem Cells International. 2015. Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1116004
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1116004