A Genomic Study of DNA Alteration Events Caused by Ionizing Radiation in Human Embryonic Stem Cells via Next-Generation Sequencing
المؤلفون المشاركون
Panyutin, Igor G.
Panyutin, Irina V.
Nguyen, Van
Neumann, Ronald D.
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-11-22
دولة النشر
مصر
عدد الصفحات
7
الملخص EN
Ionizing radiation (IR) is a known mutagen that is widely employed for medical diagnostic and therapeutic purposes.
To study the extent of genetic variations in DNA caused by IR, we used IR-sensitive human embryonic stem cells (hESCs).
Four hESC cell lines, H1, H7, H9, and H14, were subjected to IR at 0.2 or 1 Gy dose and then maintained in culture for four days before being harvested for DNA isolation.
Irradiation with 1 Gy dose resulted in significant cell death, ranging from 60% to 90% reduction in cell population.
Since IR is often implicated as a risk for inducing cancer, a primer pool targeting genomic “hotspot” regions that are frequently mutated in human cancer genes was used to generate libraries from irradiated and control samples.
Using a semiconductor-based next-generation sequencing approach, we were able to consistently sequence these samples with deep coverage for reliable data analysis.
A possible rare nucleotide variant was identified in the KIT gene (chr4:55593481) exclusively in H1 hESCs irradiated with 1 Gy dose.
More extensive further studies are warranted to assess the extent and distribution of genetic changes in hESCs after IR exposure.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Nguyen, Van& Panyutin, Irina V.& Panyutin, Igor G.& Neumann, Ronald D.. 2015. A Genomic Study of DNA Alteration Events Caused by Ionizing Radiation in Human Embryonic Stem Cells via Next-Generation Sequencing. Stem Cells International،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1116008
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Nguyen, Van…[et al.]. A Genomic Study of DNA Alteration Events Caused by Ionizing Radiation in Human Embryonic Stem Cells via Next-Generation Sequencing. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-7.
https://search.emarefa.net/detail/BIM-1116008
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Nguyen, Van& Panyutin, Irina V.& Panyutin, Igor G.& Neumann, Ronald D.. A Genomic Study of DNA Alteration Events Caused by Ionizing Radiation in Human Embryonic Stem Cells via Next-Generation Sequencing. Stem Cells International. 2015. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1116008
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1116008
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر