XPC Promotes Pluripotency of Human Dental Pulp Cells through Regulation of Oct-4Sox2c-Myc
المؤلفون المشاركون
Liu, Lu
Peng, Zhengjun
Xu, Zezhen
Wei, Xi
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-04-04
دولة النشر
مصر
عدد الصفحات
12
الملخص EN
Introduction.
Xeroderma pigmentosum group C (XPC), essential component of multisubunit stem cell coactivator complex (SCC), functions as the critical factor modulating pluripotency and genome integrity through interaction with Oct-4/Sox2.
However, its specific role in regulating pluripotency and multilineage differentiation of human dental pulp cells (DPCs) remains unknown.
Methods.
To elucidate the functional role XPC played in pluripotency and multilineage differentiation of DPCs, expressions of XPC in DPCs with long-term culture were examined by real-time PCR and western blot.
DPCs were transfected with lentiviral-mediated human XPC gene; then transfection rate was investigated by real-time PCR and western blot.
Cell cycle, apoptosis, proliferation, senescence, multilineage differentiation, and expression of Oct-4/Sox2/c-Myc in transfected DPCs were examined.
Results.
XPC, Oct-4, Sox2, and c-Myc were downregulated at P7 compared with P3 in DPCs with long-term culture.
XPC genes were upregulated in DPCs at P2 after transfection and maintained high expression level at P3 and P7.
Cell proliferation, PI value, and telomerase activity were enhanced, whereas apoptosis was suppressed in transfected DPCs.
Oct-4/Sox2/c-Myc were significantly upregulated, and multilineage differentiation in DPCs with XPC overexpression was enhanced after transfection.
Conclusions.
XPC plays an essential role in the modulation of pluripotency and multilineage differentiation of DPCs through regulation of Oct-4/Sox2/c-Myc.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Liu, Lu& Peng, Zhengjun& Xu, Zezhen& Wei, Xi. 2016. XPC Promotes Pluripotency of Human Dental Pulp Cells through Regulation of Oct-4Sox2c-Myc. Stem Cells International،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1116384
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Liu, Lu…[et al.]. XPC Promotes Pluripotency of Human Dental Pulp Cells through Regulation of Oct-4Sox2c-Myc. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1116384
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Liu, Lu& Peng, Zhengjun& Xu, Zezhen& Wei, Xi. XPC Promotes Pluripotency of Human Dental Pulp Cells through Regulation of Oct-4Sox2c-Myc. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1116384
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1116384
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر