XPC Promotes Pluripotency of Human Dental Pulp Cells through Regulation of Oct-4Sox2c-Myc
Joint Authors
Liu, Lu
Peng, Zhengjun
Xu, Zezhen
Wei, Xi
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-04-04
Country of Publication
Egypt
No. of Pages
12
Abstract EN
Introduction.
Xeroderma pigmentosum group C (XPC), essential component of multisubunit stem cell coactivator complex (SCC), functions as the critical factor modulating pluripotency and genome integrity through interaction with Oct-4/Sox2.
However, its specific role in regulating pluripotency and multilineage differentiation of human dental pulp cells (DPCs) remains unknown.
Methods.
To elucidate the functional role XPC played in pluripotency and multilineage differentiation of DPCs, expressions of XPC in DPCs with long-term culture were examined by real-time PCR and western blot.
DPCs were transfected with lentiviral-mediated human XPC gene; then transfection rate was investigated by real-time PCR and western blot.
Cell cycle, apoptosis, proliferation, senescence, multilineage differentiation, and expression of Oct-4/Sox2/c-Myc in transfected DPCs were examined.
Results.
XPC, Oct-4, Sox2, and c-Myc were downregulated at P7 compared with P3 in DPCs with long-term culture.
XPC genes were upregulated in DPCs at P2 after transfection and maintained high expression level at P3 and P7.
Cell proliferation, PI value, and telomerase activity were enhanced, whereas apoptosis was suppressed in transfected DPCs.
Oct-4/Sox2/c-Myc were significantly upregulated, and multilineage differentiation in DPCs with XPC overexpression was enhanced after transfection.
Conclusions.
XPC plays an essential role in the modulation of pluripotency and multilineage differentiation of DPCs through regulation of Oct-4/Sox2/c-Myc.
American Psychological Association (APA)
Liu, Lu& Peng, Zhengjun& Xu, Zezhen& Wei, Xi. 2016. XPC Promotes Pluripotency of Human Dental Pulp Cells through Regulation of Oct-4Sox2c-Myc. Stem Cells International،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1116384
Modern Language Association (MLA)
Liu, Lu…[et al.]. XPC Promotes Pluripotency of Human Dental Pulp Cells through Regulation of Oct-4Sox2c-Myc. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1116384
American Medical Association (AMA)
Liu, Lu& Peng, Zhengjun& Xu, Zezhen& Wei, Xi. XPC Promotes Pluripotency of Human Dental Pulp Cells through Regulation of Oct-4Sox2c-Myc. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1116384
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1116384