Er-Xian Decoction Stimulates Osteoblastic Differentiation of Bone Mesenchymal Stem Cells in Ovariectomized Mice and Its Gene Profile Analysis
المؤلفون المشاركون
Liu, Shu-Fen
Bian, Qin
Wang, Yongjun
Huang, Jianhua
Wang, Jing
Zhao, Yongjian
Lu, Sheng
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-03-15
دولة النشر
مصر
عدد الصفحات
10
الملخص EN
We studied the bone mesenchymal stem cells (bMSCs) and gene profiles regulated by Er-Xian Decoction (EXD), a traditional Chinese herbal formula widely used for postmenopausal osteoporosis treatment.
Six-month-old female Imprinting Control Region mice that underwent ovariectomy were treated with EXD.
After 3 months, bone mass was evaluated by μCT and histological and immunohistochemical detection.
The self-renewal and differentiation capacities of bMSCs were evaluated by colony-forming unit-fibroblastic, colony-forming unit-adipocyte, and alkaline phosphatase staining.
In addition, the expression of 26991 genes of bMSCs ex vivo at 2 weeks after EXD-treatment or of bMSCs in vitro after exposure to conditioned serum from EXD-treated rats was measured and analyzed using NimbleGen Gene Expression Profiling and Cluster and pathway analysis.
EXD treatment increased bone mass, elevating osteocalcin protein levels in vivo and facilitating the self-renewal and osteoblastic differentiation of bMSCs ex vivo.
EXD rescued several gene expressions that were dysregulated by OVX.
These genes overlapped and their functions were involved in ten pathways between ex vivo and in vitro experiments.
EXD exerts an osteogenic effect on bMSCs in OVX induced osteoporotic mice.
Our results contribute to further study of its molecular mechanism and traditional use in the treatment of postmenopausal osteoporosis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Liu, Shu-Fen& Huang, Jianhua& Wang, Jing& Zhao, Yongjian& Lu, Sheng& Wang, Yongjun…[et al.]. 2016. Er-Xian Decoction Stimulates Osteoblastic Differentiation of Bone Mesenchymal Stem Cells in Ovariectomized Mice and Its Gene Profile Analysis. Stem Cells International،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1116494
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Liu, Shu-Fen…[et al.]. Er-Xian Decoction Stimulates Osteoblastic Differentiation of Bone Mesenchymal Stem Cells in Ovariectomized Mice and Its Gene Profile Analysis. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1116494
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Liu, Shu-Fen& Huang, Jianhua& Wang, Jing& Zhao, Yongjian& Lu, Sheng& Wang, Yongjun…[et al.]. Er-Xian Decoction Stimulates Osteoblastic Differentiation of Bone Mesenchymal Stem Cells in Ovariectomized Mice and Its Gene Profile Analysis. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1116494
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1116494
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر