Er-Xian Decoction Stimulates Osteoblastic Differentiation of Bone Mesenchymal Stem Cells in Ovariectomized Mice and Its Gene Profile Analysis
Joint Authors
Liu, Shu-Fen
Bian, Qin
Wang, Yongjun
Huang, Jianhua
Wang, Jing
Zhao, Yongjian
Lu, Sheng
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-03-15
Country of Publication
Egypt
No. of Pages
10
Abstract EN
We studied the bone mesenchymal stem cells (bMSCs) and gene profiles regulated by Er-Xian Decoction (EXD), a traditional Chinese herbal formula widely used for postmenopausal osteoporosis treatment.
Six-month-old female Imprinting Control Region mice that underwent ovariectomy were treated with EXD.
After 3 months, bone mass was evaluated by μCT and histological and immunohistochemical detection.
The self-renewal and differentiation capacities of bMSCs were evaluated by colony-forming unit-fibroblastic, colony-forming unit-adipocyte, and alkaline phosphatase staining.
In addition, the expression of 26991 genes of bMSCs ex vivo at 2 weeks after EXD-treatment or of bMSCs in vitro after exposure to conditioned serum from EXD-treated rats was measured and analyzed using NimbleGen Gene Expression Profiling and Cluster and pathway analysis.
EXD treatment increased bone mass, elevating osteocalcin protein levels in vivo and facilitating the self-renewal and osteoblastic differentiation of bMSCs ex vivo.
EXD rescued several gene expressions that were dysregulated by OVX.
These genes overlapped and their functions were involved in ten pathways between ex vivo and in vitro experiments.
EXD exerts an osteogenic effect on bMSCs in OVX induced osteoporotic mice.
Our results contribute to further study of its molecular mechanism and traditional use in the treatment of postmenopausal osteoporosis.
American Psychological Association (APA)
Liu, Shu-Fen& Huang, Jianhua& Wang, Jing& Zhao, Yongjian& Lu, Sheng& Wang, Yongjun…[et al.]. 2016. Er-Xian Decoction Stimulates Osteoblastic Differentiation of Bone Mesenchymal Stem Cells in Ovariectomized Mice and Its Gene Profile Analysis. Stem Cells International،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1116494
Modern Language Association (MLA)
Liu, Shu-Fen…[et al.]. Er-Xian Decoction Stimulates Osteoblastic Differentiation of Bone Mesenchymal Stem Cells in Ovariectomized Mice and Its Gene Profile Analysis. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1116494
American Medical Association (AMA)
Liu, Shu-Fen& Huang, Jianhua& Wang, Jing& Zhao, Yongjian& Lu, Sheng& Wang, Yongjun…[et al.]. Er-Xian Decoction Stimulates Osteoblastic Differentiation of Bone Mesenchymal Stem Cells in Ovariectomized Mice and Its Gene Profile Analysis. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1116494
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1116494