Subculture of Germ Cell-Derived Colonies with GATA4-Positive Feeder Cells from Neonatal Pig Testes
المؤلفون المشاركون
Park, Chankyu
Kim, Jin-Hoi
Song, Hyuk
Do, Jeong-Tae
Lee, Kyung Hoon
Lee, Won Young
Kim, Jae Hwan
Choi, Young Suk
Kim, Nam-Hyung
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-01-06
دولة النشر
مصر
عدد الصفحات
9
الملخص EN
Enrichment of spermatogonial stem cells is important for studying their self-renewal and differentiation.
Although germ cell-derived colonies (GDCs) have been successfully cultured from neonatal pig testicular cells under 31°C conditions, the short period of in vitro maintenance (<2 months) limited their application to further investigations.
To develop a culture method that allows for in vitro maintenance of GDCs for long periods, we subcultured the GDCs with freshly prepared somatic cells from neonatal pig testes as feeder cells.
The subcultured GDCs were maintained up to passage 13 with the fresh feeder cells (FFCs) and then frozen.
Eight months later, the frozen GDCs could again form the colonies on FFCs as shown in passages 1 to 13.
Immunocytochemistry data revealed that the FFCs expressed GATA-binding protein 4 (GATA4), which is also detected in the cells of neonatal testes and total testicular cells, and that the expression of GATA4 was decreased in used old feeder cells.
The subcultured GDCs in each passage had germ and stem cell characteristics, and flow cytometric analyses revealed that ~60% of these cells were GFRα-1 positive.
In conclusion, neonatal pig testes-derived GDCs can be maintained for long periods with GATA4-expressing testicular somatic cells.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Lee, Kyung Hoon& Lee, Won Young& Kim, Jin-Hoi& Park, Chankyu& Do, Jeong-Tae& Kim, Jae Hwan…[et al.]. 2016. Subculture of Germ Cell-Derived Colonies with GATA4-Positive Feeder Cells from Neonatal Pig Testes. Stem Cells International،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1116863
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Lee, Kyung Hoon…[et al.]. Subculture of Germ Cell-Derived Colonies with GATA4-Positive Feeder Cells from Neonatal Pig Testes. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1116863
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Lee, Kyung Hoon& Lee, Won Young& Kim, Jin-Hoi& Park, Chankyu& Do, Jeong-Tae& Kim, Jae Hwan…[et al.]. Subculture of Germ Cell-Derived Colonies with GATA4-Positive Feeder Cells from Neonatal Pig Testes. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1116863
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1116863
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر