Subculture of Germ Cell-Derived Colonies with GATA4-Positive Feeder Cells from Neonatal Pig Testes
Joint Authors
Park, Chankyu
Kim, Jin-Hoi
Song, Hyuk
Do, Jeong-Tae
Lee, Kyung Hoon
Lee, Won Young
Kim, Jae Hwan
Choi, Young Suk
Kim, Nam-Hyung
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-01-06
Country of Publication
Egypt
No. of Pages
9
Abstract EN
Enrichment of spermatogonial stem cells is important for studying their self-renewal and differentiation.
Although germ cell-derived colonies (GDCs) have been successfully cultured from neonatal pig testicular cells under 31°C conditions, the short period of in vitro maintenance (<2 months) limited their application to further investigations.
To develop a culture method that allows for in vitro maintenance of GDCs for long periods, we subcultured the GDCs with freshly prepared somatic cells from neonatal pig testes as feeder cells.
The subcultured GDCs were maintained up to passage 13 with the fresh feeder cells (FFCs) and then frozen.
Eight months later, the frozen GDCs could again form the colonies on FFCs as shown in passages 1 to 13.
Immunocytochemistry data revealed that the FFCs expressed GATA-binding protein 4 (GATA4), which is also detected in the cells of neonatal testes and total testicular cells, and that the expression of GATA4 was decreased in used old feeder cells.
The subcultured GDCs in each passage had germ and stem cell characteristics, and flow cytometric analyses revealed that ~60% of these cells were GFRα-1 positive.
In conclusion, neonatal pig testes-derived GDCs can be maintained for long periods with GATA4-expressing testicular somatic cells.
American Psychological Association (APA)
Lee, Kyung Hoon& Lee, Won Young& Kim, Jin-Hoi& Park, Chankyu& Do, Jeong-Tae& Kim, Jae Hwan…[et al.]. 2016. Subculture of Germ Cell-Derived Colonies with GATA4-Positive Feeder Cells from Neonatal Pig Testes. Stem Cells International،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1116863
Modern Language Association (MLA)
Lee, Kyung Hoon…[et al.]. Subculture of Germ Cell-Derived Colonies with GATA4-Positive Feeder Cells from Neonatal Pig Testes. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1116863
American Medical Association (AMA)
Lee, Kyung Hoon& Lee, Won Young& Kim, Jin-Hoi& Park, Chankyu& Do, Jeong-Tae& Kim, Jae Hwan…[et al.]. Subculture of Germ Cell-Derived Colonies with GATA4-Positive Feeder Cells from Neonatal Pig Testes. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1116863
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1116863