Establishment of a Novel Model for Anticancer Drug Resistance in Three-Dimensional Primary Culture of Tumor Microenvironment
المؤلفون المشاركون
Usui, Tatsuya
Sakurai, Masashi
Enjoji, Shuhei
Kawasaki, Hideyoshi
Umata, Koji
Ohama, Takashi
Fujiwara, Nobuyuki
Yabe, Ryotaro
Tsuji, Shunya
Yamawaki, Hideyuki
Hazama, Shoichi
Takenouchi, Hiroko
Nakajima, Masao
Tsunedomi, Ryouichi
Suzuki, Nobuaki
Nagano, Hiroaki
Sato, Koichi
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-12-29
دولة النشر
مصر
عدد الصفحات
10
الملخص EN
Tumor microenvironment has been implicated in tumor development and progression.
As a three-dimensional tumor microenvironment model, air liquid interface (ALI) organoid culture from oncogene transgenic mouse gastrointestinal tissues was recently produced.
However, ALI organoid culture system from tissues of colorectal cancer patients has not been established.
Here, we developed an ALI organoid model from normal and tumor colorectal tissues of human patients.
Both organoids were successfully generated and showed cystic structures containing an epithelial layer and surrounding mesenchymal stromal cells.
Structures of tumor organoids closely resembled primary tumor epithelium.
Expression of an epithelial cell marker, E-cadherin, a goblet cell marker, MUC2, and a fibroblast marker, vimentin, but not a myofibroblast marker, α-smooth muscle actin (SMA), was observed in normal organoids.
Expression of E-cadherin, MUC2, vimentin, and α-SMA was observed in tumor organoids.
Expression of a cancer stem cell marker, LGR5 in tumor organoids, was higher than that in primary tumor tissues.
Tumor organoids were more resistant to toxicity of 5-fluorouracil and Irinotecan than colorectal cancer cell lines, SW480, SW620, and HCT116.
These findings indicate that ALI organoid culture from colorectal cancer patients may become a novel model that is useful for examining resistance to chemotherapy in tumor microenvironment.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Usui, Tatsuya& Sakurai, Masashi& Enjoji, Shuhei& Kawasaki, Hideyoshi& Umata, Koji& Ohama, Takashi…[et al.]. 2016. Establishment of a Novel Model for Anticancer Drug Resistance in Three-Dimensional Primary Culture of Tumor Microenvironment. Stem Cells International،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1117025
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Usui, Tatsuya…[et al.]. Establishment of a Novel Model for Anticancer Drug Resistance in Three-Dimensional Primary Culture of Tumor Microenvironment. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1117025
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Usui, Tatsuya& Sakurai, Masashi& Enjoji, Shuhei& Kawasaki, Hideyoshi& Umata, Koji& Ohama, Takashi…[et al.]. Establishment of a Novel Model for Anticancer Drug Resistance in Three-Dimensional Primary Culture of Tumor Microenvironment. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1117025
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1117025
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر