Establishment of a Novel Model for Anticancer Drug Resistance in Three-Dimensional Primary Culture of Tumor Microenvironment
Joint Authors
Usui, Tatsuya
Sakurai, Masashi
Enjoji, Shuhei
Kawasaki, Hideyoshi
Umata, Koji
Ohama, Takashi
Fujiwara, Nobuyuki
Yabe, Ryotaro
Tsuji, Shunya
Yamawaki, Hideyuki
Hazama, Shoichi
Takenouchi, Hiroko
Nakajima, Masao
Tsunedomi, Ryouichi
Suzuki, Nobuaki
Nagano, Hiroaki
Sato, Koichi
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-12-29
Country of Publication
Egypt
No. of Pages
10
Abstract EN
Tumor microenvironment has been implicated in tumor development and progression.
As a three-dimensional tumor microenvironment model, air liquid interface (ALI) organoid culture from oncogene transgenic mouse gastrointestinal tissues was recently produced.
However, ALI organoid culture system from tissues of colorectal cancer patients has not been established.
Here, we developed an ALI organoid model from normal and tumor colorectal tissues of human patients.
Both organoids were successfully generated and showed cystic structures containing an epithelial layer and surrounding mesenchymal stromal cells.
Structures of tumor organoids closely resembled primary tumor epithelium.
Expression of an epithelial cell marker, E-cadherin, a goblet cell marker, MUC2, and a fibroblast marker, vimentin, but not a myofibroblast marker, α-smooth muscle actin (SMA), was observed in normal organoids.
Expression of E-cadherin, MUC2, vimentin, and α-SMA was observed in tumor organoids.
Expression of a cancer stem cell marker, LGR5 in tumor organoids, was higher than that in primary tumor tissues.
Tumor organoids were more resistant to toxicity of 5-fluorouracil and Irinotecan than colorectal cancer cell lines, SW480, SW620, and HCT116.
These findings indicate that ALI organoid culture from colorectal cancer patients may become a novel model that is useful for examining resistance to chemotherapy in tumor microenvironment.
American Psychological Association (APA)
Usui, Tatsuya& Sakurai, Masashi& Enjoji, Shuhei& Kawasaki, Hideyoshi& Umata, Koji& Ohama, Takashi…[et al.]. 2016. Establishment of a Novel Model for Anticancer Drug Resistance in Three-Dimensional Primary Culture of Tumor Microenvironment. Stem Cells International،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1117025
Modern Language Association (MLA)
Usui, Tatsuya…[et al.]. Establishment of a Novel Model for Anticancer Drug Resistance in Three-Dimensional Primary Culture of Tumor Microenvironment. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1117025
American Medical Association (AMA)
Usui, Tatsuya& Sakurai, Masashi& Enjoji, Shuhei& Kawasaki, Hideyoshi& Umata, Koji& Ohama, Takashi…[et al.]. Establishment of a Novel Model for Anticancer Drug Resistance in Three-Dimensional Primary Culture of Tumor Microenvironment. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1117025
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1117025