Allogeneic Platelet Releasate Preparations Derived via a Novel Rapid Thrombin Activation Process Promote Rapid Growth and Increased BMP-2 and BMP-4 Expression in Human Adipose-Derived Stem Cells
المؤلفون المشاركون
McLaughlin, Michael
Gagnet, Paul
Cunningham, Elizabeth
Yeager, Randi
D’Amico, Michael
Guski, Katie
Scarpone, Michael
Kuebler, Daniel
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-12-28
دولة النشر
مصر
عدد الصفحات
9
الملخص EN
The administration of human adipose-derived stem cells (ASCs) represents a promising regenerative therapy for the treatment of orthopedic injuries.
While ASCs can be easily isolated from liposuction-derived adipose tissue, most clinical applications will likely require in vitro culture expansion of these cells using nonxenogeneic components.
In this study, platelet releasate was generated using a novel rapid thrombin activation method (tPR).
ASCs grown in media supplemented with tPR proliferated much faster than ASCs grown in media supplemented with 10% fetal bovine serum.
The cells also retained the ability to differentiate along chondrogenic, adipogenic, and osteogenic lineages.
The tPR cultured ASCs displayed elevated expression of BMP-4 (5.7 ± 0.97-fold increase) and BMP-2 (4.7 ± 1.3-fold increase) and decreased expression of PDGF-B (4.0 ± 1.4-fold decrease) and FGF-2 (33 ± 9.0-fold decrease).
No significant changes in expression were seen with TGF-β and VEGF.
This pattern of gene expression was consistent across different allogeneic tPR samples and different ASC lines.
The use of allogeneic rapidly activated tPR to culture ASCs is associated with both an increased cell yield and a defined gene expression profile making it an attractive option for cell expansion prior to cell-based therapy for orthopedic applications.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
McLaughlin, Michael& Gagnet, Paul& Cunningham, Elizabeth& Yeager, Randi& D’Amico, Michael& Guski, Katie…[et al.]. 2015. Allogeneic Platelet Releasate Preparations Derived via a Novel Rapid Thrombin Activation Process Promote Rapid Growth and Increased BMP-2 and BMP-4 Expression in Human Adipose-Derived Stem Cells. Stem Cells International،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1117073
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
McLaughlin, Michael…[et al.]. Allogeneic Platelet Releasate Preparations Derived via a Novel Rapid Thrombin Activation Process Promote Rapid Growth and Increased BMP-2 and BMP-4 Expression in Human Adipose-Derived Stem Cells. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1117073
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
McLaughlin, Michael& Gagnet, Paul& Cunningham, Elizabeth& Yeager, Randi& D’Amico, Michael& Guski, Katie…[et al.]. Allogeneic Platelet Releasate Preparations Derived via a Novel Rapid Thrombin Activation Process Promote Rapid Growth and Increased BMP-2 and BMP-4 Expression in Human Adipose-Derived Stem Cells. Stem Cells International. 2015. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1117073
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1117073
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر