Allogeneic Platelet Releasate Preparations Derived via a Novel Rapid Thrombin Activation Process Promote Rapid Growth and Increased BMP-2 and BMP-4 Expression in Human Adipose-Derived Stem Cells
Joint Authors
McLaughlin, Michael
Gagnet, Paul
Cunningham, Elizabeth
Yeager, Randi
D’Amico, Michael
Guski, Katie
Scarpone, Michael
Kuebler, Daniel
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-12-28
Country of Publication
Egypt
No. of Pages
9
Abstract EN
The administration of human adipose-derived stem cells (ASCs) represents a promising regenerative therapy for the treatment of orthopedic injuries.
While ASCs can be easily isolated from liposuction-derived adipose tissue, most clinical applications will likely require in vitro culture expansion of these cells using nonxenogeneic components.
In this study, platelet releasate was generated using a novel rapid thrombin activation method (tPR).
ASCs grown in media supplemented with tPR proliferated much faster than ASCs grown in media supplemented with 10% fetal bovine serum.
The cells also retained the ability to differentiate along chondrogenic, adipogenic, and osteogenic lineages.
The tPR cultured ASCs displayed elevated expression of BMP-4 (5.7 ± 0.97-fold increase) and BMP-2 (4.7 ± 1.3-fold increase) and decreased expression of PDGF-B (4.0 ± 1.4-fold decrease) and FGF-2 (33 ± 9.0-fold decrease).
No significant changes in expression were seen with TGF-β and VEGF.
This pattern of gene expression was consistent across different allogeneic tPR samples and different ASC lines.
The use of allogeneic rapidly activated tPR to culture ASCs is associated with both an increased cell yield and a defined gene expression profile making it an attractive option for cell expansion prior to cell-based therapy for orthopedic applications.
American Psychological Association (APA)
McLaughlin, Michael& Gagnet, Paul& Cunningham, Elizabeth& Yeager, Randi& D’Amico, Michael& Guski, Katie…[et al.]. 2015. Allogeneic Platelet Releasate Preparations Derived via a Novel Rapid Thrombin Activation Process Promote Rapid Growth and Increased BMP-2 and BMP-4 Expression in Human Adipose-Derived Stem Cells. Stem Cells International،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1117073
Modern Language Association (MLA)
McLaughlin, Michael…[et al.]. Allogeneic Platelet Releasate Preparations Derived via a Novel Rapid Thrombin Activation Process Promote Rapid Growth and Increased BMP-2 and BMP-4 Expression in Human Adipose-Derived Stem Cells. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1117073
American Medical Association (AMA)
McLaughlin, Michael& Gagnet, Paul& Cunningham, Elizabeth& Yeager, Randi& D’Amico, Michael& Guski, Katie…[et al.]. Allogeneic Platelet Releasate Preparations Derived via a Novel Rapid Thrombin Activation Process Promote Rapid Growth and Increased BMP-2 and BMP-4 Expression in Human Adipose-Derived Stem Cells. Stem Cells International. 2015. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1117073
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1117073