Induction of Tenogenic Differentiation Mediated by Extracellular Tendon Matrix and Short-Term Cyclic Stretching
المؤلفون المشاركون
Kasper, Cornelia
Brehm, Walter
Burk, Janina
Plenge, Amelie
Heller, Sandra
Pfeiffer, Bastian
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-08-18
دولة النشر
مصر
عدد الصفحات
11
الملخص EN
Tendon and ligament pathologies are still a therapeutic challenge, due to the difficulty in restoring the complex extracellular matrix architecture and biomechanical strength.
While progress is being made in cell-based therapies and tissue engineering approaches, comprehensive understanding of the fate of progenitor cells in tendon healing is still lacking.
The aim of this study was to investigate the effect of decellularized tendon matrix and moderate cyclic stretching as natural stimuli which could potentially direct tenogenic fate.
Equine adipose-derived mesenchymal stromal cells (MSC) were seeded on decellularized tendon matrix scaffolds.
Mechanical stimulation was applied in a custom-made cyclic strain bioreactor.
Assessment was performed 4 h, 8 h, and 24 h following mechanical stimulation.
Scaffold culture induced cell alignment and changes in expression of tendon-related genes, although cell viability was decreased compared to monolayer culture.
Short mechanical stimulation periods enhanced most of the scaffold-induced effects.
Collagen 1A2 expression levels were decreased, while collagen 3A1 and decorin levels were increased.
Tenascin-C and scleraxis expression showed an initial decrease but had increased 24 h after stimulation.
The results obtained suggest that decellularized tendon matrix, supported by cyclic stretching, can induce tenogenic differentiation and the synthesis of tendon components important for matrix remodeling.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Burk, Janina& Plenge, Amelie& Brehm, Walter& Heller, Sandra& Pfeiffer, Bastian& Kasper, Cornelia. 2016. Induction of Tenogenic Differentiation Mediated by Extracellular Tendon Matrix and Short-Term Cyclic Stretching. Stem Cells International،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1117096
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Burk, Janina…[et al.]. Induction of Tenogenic Differentiation Mediated by Extracellular Tendon Matrix and Short-Term Cyclic Stretching. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1117096
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Burk, Janina& Plenge, Amelie& Brehm, Walter& Heller, Sandra& Pfeiffer, Bastian& Kasper, Cornelia. Induction of Tenogenic Differentiation Mediated by Extracellular Tendon Matrix and Short-Term Cyclic Stretching. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1117096
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1117096
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر