Induction of Tenogenic Differentiation Mediated by Extracellular Tendon Matrix and Short-Term Cyclic Stretching

Joint Authors

Kasper, Cornelia
Brehm, Walter
Burk, Janina
Plenge, Amelie
Heller, Sandra
Pfeiffer, Bastian

Source

Stem Cells International

Issue

Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-11, 11 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2016-08-18

Country of Publication

Egypt

No. of Pages

11

Abstract EN

Tendon and ligament pathologies are still a therapeutic challenge, due to the difficulty in restoring the complex extracellular matrix architecture and biomechanical strength.

While progress is being made in cell-based therapies and tissue engineering approaches, comprehensive understanding of the fate of progenitor cells in tendon healing is still lacking.

The aim of this study was to investigate the effect of decellularized tendon matrix and moderate cyclic stretching as natural stimuli which could potentially direct tenogenic fate.

Equine adipose-derived mesenchymal stromal cells (MSC) were seeded on decellularized tendon matrix scaffolds.

Mechanical stimulation was applied in a custom-made cyclic strain bioreactor.

Assessment was performed 4 h, 8 h, and 24 h following mechanical stimulation.

Scaffold culture induced cell alignment and changes in expression of tendon-related genes, although cell viability was decreased compared to monolayer culture.

Short mechanical stimulation periods enhanced most of the scaffold-induced effects.

Collagen 1A2 expression levels were decreased, while collagen 3A1 and decorin levels were increased.

Tenascin-C and scleraxis expression showed an initial decrease but had increased 24 h after stimulation.

The results obtained suggest that decellularized tendon matrix, supported by cyclic stretching, can induce tenogenic differentiation and the synthesis of tendon components important for matrix remodeling.

American Psychological Association (APA)

Burk, Janina& Plenge, Amelie& Brehm, Walter& Heller, Sandra& Pfeiffer, Bastian& Kasper, Cornelia. 2016. Induction of Tenogenic Differentiation Mediated by Extracellular Tendon Matrix and Short-Term Cyclic Stretching. Stem Cells International،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1117096

Modern Language Association (MLA)

Burk, Janina…[et al.]. Induction of Tenogenic Differentiation Mediated by Extracellular Tendon Matrix and Short-Term Cyclic Stretching. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1117096

American Medical Association (AMA)

Burk, Janina& Plenge, Amelie& Brehm, Walter& Heller, Sandra& Pfeiffer, Bastian& Kasper, Cornelia. Induction of Tenogenic Differentiation Mediated by Extracellular Tendon Matrix and Short-Term Cyclic Stretching. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1117096

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1117096