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Platelet-Rich Plasma Obtained with Different Anticoagulants and Their Effect on Platelet Numbers and Mesenchymal Stromal Cells Behavior In Vitro
المؤلفون المشاركون
do Amaral, Ronaldo José Farias Corrêa
da Silva, Nemias Pereira
Haddad, Natália Ferreira
Lopes, Luana Siqueira
Ferreira, Fábio Dias
Filho, Ricardo Bastos
Cappelletti, Paola Alejandra
de Mello, Wallace
Cordeiro-Spinetti, Eric
Balduino, Alex
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-06-02
دولة النشر
مصر
عدد الصفحات
11
الملخص EN
There are promising results in the use of platelet-rich plasma (PRP) for musculoskeletal tissue repair.
However, the variability in the methodology for its obtaining may cause different and opposing findings in the literature.
Particularly, the choice of the anticoagulant is the first definition to be made.
In this work, blood was collected with sodium citrate (SC), ethylenediaminetetraacetic acid (EDTA), or anticoagulant citrate dextrose (ACD) solution A, as anticoagulants, prior to PRP obtaining.
Hematological analysis and growth factors release quantification were performed, and the effects on mesenchymal stromal cell (MSC) culture, such as cytotoxicity and cell proliferation (evaluated by MTT method) and gene expression, were evaluated.
The use of EDTA resulted in higher platelet yield in whole blood; however, it induced an increase in the mean platelet volume (MPV) following the blood centrifugation steps for PRP obtaining.
The use of SC and ACD resulted in higher induction of MSC proliferation.
On the other hand, PRP obtained in SC presented the higher platelet recovery after the blood first centrifugation step and a minimal change in MSC gene expression.
Therefore, we suggest the use of SC as the anticoagulant for PRP obtaining.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
do Amaral, Ronaldo José Farias Corrêa& da Silva, Nemias Pereira& Haddad, Natália Ferreira& Lopes, Luana Siqueira& Ferreira, Fábio Dias& Filho, Ricardo Bastos…[et al.]. 2016. Platelet-Rich Plasma Obtained with Different Anticoagulants and Their Effect on Platelet Numbers and Mesenchymal Stromal Cells Behavior In Vitro. Stem Cells International،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1117109
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
do Amaral, Ronaldo José Farias Corrêa…[et al.]. Platelet-Rich Plasma Obtained with Different Anticoagulants and Their Effect on Platelet Numbers and Mesenchymal Stromal Cells Behavior In Vitro. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1117109
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
do Amaral, Ronaldo José Farias Corrêa& da Silva, Nemias Pereira& Haddad, Natália Ferreira& Lopes, Luana Siqueira& Ferreira, Fábio Dias& Filho, Ricardo Bastos…[et al.]. Platelet-Rich Plasma Obtained with Different Anticoagulants and Their Effect on Platelet Numbers and Mesenchymal Stromal Cells Behavior In Vitro. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1117109
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1117109
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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