Platelet-Rich Plasma Obtained with Different Anticoagulants and Their Effect on Platelet Numbers and Mesenchymal Stromal Cells Behavior In Vitro
Joint Authors
do Amaral, Ronaldo José Farias Corrêa
da Silva, Nemias Pereira
Haddad, Natália Ferreira
Lopes, Luana Siqueira
Ferreira, Fábio Dias
Filho, Ricardo Bastos
Cappelletti, Paola Alejandra
de Mello, Wallace
Cordeiro-Spinetti, Eric
Balduino, Alex
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-06-02
Country of Publication
Egypt
No. of Pages
11
Abstract EN
There are promising results in the use of platelet-rich plasma (PRP) for musculoskeletal tissue repair.
However, the variability in the methodology for its obtaining may cause different and opposing findings in the literature.
Particularly, the choice of the anticoagulant is the first definition to be made.
In this work, blood was collected with sodium citrate (SC), ethylenediaminetetraacetic acid (EDTA), or anticoagulant citrate dextrose (ACD) solution A, as anticoagulants, prior to PRP obtaining.
Hematological analysis and growth factors release quantification were performed, and the effects on mesenchymal stromal cell (MSC) culture, such as cytotoxicity and cell proliferation (evaluated by MTT method) and gene expression, were evaluated.
The use of EDTA resulted in higher platelet yield in whole blood; however, it induced an increase in the mean platelet volume (MPV) following the blood centrifugation steps for PRP obtaining.
The use of SC and ACD resulted in higher induction of MSC proliferation.
On the other hand, PRP obtained in SC presented the higher platelet recovery after the blood first centrifugation step and a minimal change in MSC gene expression.
Therefore, we suggest the use of SC as the anticoagulant for PRP obtaining.
American Psychological Association (APA)
do Amaral, Ronaldo José Farias Corrêa& da Silva, Nemias Pereira& Haddad, Natália Ferreira& Lopes, Luana Siqueira& Ferreira, Fábio Dias& Filho, Ricardo Bastos…[et al.]. 2016. Platelet-Rich Plasma Obtained with Different Anticoagulants and Their Effect on Platelet Numbers and Mesenchymal Stromal Cells Behavior In Vitro. Stem Cells International،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1117109
Modern Language Association (MLA)
do Amaral, Ronaldo José Farias Corrêa…[et al.]. Platelet-Rich Plasma Obtained with Different Anticoagulants and Their Effect on Platelet Numbers and Mesenchymal Stromal Cells Behavior In Vitro. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1117109
American Medical Association (AMA)
do Amaral, Ronaldo José Farias Corrêa& da Silva, Nemias Pereira& Haddad, Natália Ferreira& Lopes, Luana Siqueira& Ferreira, Fábio Dias& Filho, Ricardo Bastos…[et al.]. Platelet-Rich Plasma Obtained with Different Anticoagulants and Their Effect on Platelet Numbers and Mesenchymal Stromal Cells Behavior In Vitro. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1117109
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1117109