The Rise of CRISPRCas for Genome Editing in Stem Cells

المؤلفون المشاركون

Shui, Bing
Hernandez Matias, Liz
Guo, Yi
Peng, Ying

المصدر

Stem Cells International

العدد

المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-17، 17ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2016-01-06

دولة النشر

مصر

عدد الصفحات

17

الملخص EN

Genetic manipulation is a powerful tool to establish the causal relationship between a genetic lesion and a particular pathological phenotype.

The rise of CRISPR/Cas9 genome-engineering tools overcame the traditional technical bottleneck for routine site-specific genetic manipulation in cells.

To create the perfect in vitro cell model, there is significant interest from the stem cell research community to adopt this fast evolving technology.

This review addresses this need directly by providing both the up-to-date biochemical rationale of CRISPR-mediated genome engineering and detailed practical guidelines for the design and execution of CRISPR experiments in cell models.

Ultimately, this review will serve as a timely and comprehensive guide for this fast developing technology.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Shui, Bing& Hernandez Matias, Liz& Guo, Yi& Peng, Ying. 2016. The Rise of CRISPRCas for Genome Editing in Stem Cells. Stem Cells International،Vol. 2016, no. 2016, pp.1-17.
https://search.emarefa.net/detail/BIM-1117198

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Shui, Bing…[et al.]. The Rise of CRISPRCas for Genome Editing in Stem Cells. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-17.
https://search.emarefa.net/detail/BIM-1117198

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Shui, Bing& Hernandez Matias, Liz& Guo, Yi& Peng, Ying. The Rise of CRISPRCas for Genome Editing in Stem Cells. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-17.
https://search.emarefa.net/detail/BIM-1117198

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1117198