The Rise of CRISPRCas for Genome Editing in Stem Cells
Joint Authors
Shui, Bing
Hernandez Matias, Liz
Guo, Yi
Peng, Ying
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-01-06
Country of Publication
Egypt
No. of Pages
17
Abstract EN
Genetic manipulation is a powerful tool to establish the causal relationship between a genetic lesion and a particular pathological phenotype.
The rise of CRISPR/Cas9 genome-engineering tools overcame the traditional technical bottleneck for routine site-specific genetic manipulation in cells.
To create the perfect in vitro cell model, there is significant interest from the stem cell research community to adopt this fast evolving technology.
This review addresses this need directly by providing both the up-to-date biochemical rationale of CRISPR-mediated genome engineering and detailed practical guidelines for the design and execution of CRISPR experiments in cell models.
Ultimately, this review will serve as a timely and comprehensive guide for this fast developing technology.
American Psychological Association (APA)
Shui, Bing& Hernandez Matias, Liz& Guo, Yi& Peng, Ying. 2016. The Rise of CRISPRCas for Genome Editing in Stem Cells. Stem Cells International،Vol. 2016, no. 2016, pp.1-17.
https://search.emarefa.net/detail/BIM-1117198
Modern Language Association (MLA)
Shui, Bing…[et al.]. The Rise of CRISPRCas for Genome Editing in Stem Cells. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-17.
https://search.emarefa.net/detail/BIM-1117198
American Medical Association (AMA)
Shui, Bing& Hernandez Matias, Liz& Guo, Yi& Peng, Ying. The Rise of CRISPRCas for Genome Editing in Stem Cells. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-17.
https://search.emarefa.net/detail/BIM-1117198
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1117198