Fibronectin and Cyclic Strain Improve Cardiac Progenitor Cell Regenerative Potential In Vitro
المؤلفون المشاركون
French, Kristin M.
Maxwell, Joshua T.
Bhutani, Srishti
Ghosh-Choudhary, Shohini
Fierro, Marcos J.
Johnson, Todd D.
Christman, Karen L.
Taylor, W. Robert
Davis, Michael E.
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-08-16
دولة النشر
مصر
عدد الصفحات
11
الملخص EN
Cardiac progenitor cells (CPCs) have rapidly advanced to clinical trials, yet little is known regarding their interaction with the microenvironment.
Signaling cues present in the microenvironment change with development and disease.
This work aims to assess the influence of two distinct signaling moieties on CPCs: cyclic biaxial strain and extracellular matrix.
We evaluate four endpoints for improving CPC therapy: paracrine signaling, proliferation, connexin43 expression, and alignment.
Vascular endothelial growth factor A (about 900 pg/mL) was secreted by CPCs cultured on fibronectin and collagen I.
The application of mechanical strain increased vascular endothelial growth factor A secretion 2–4-fold for CPCs cultured on poly-L-lysine, laminin, or a naturally derived cardiac extracellular matrix.
CPC proliferation was at least 25% higher on fibronectin than that on other matrices, especially for lower strain magnitudes.
At 5% strain, connexin43 expression was highest on fibronectin.
With increasing strain magnitude, connexin43 expression decreased by as much as 60% in CPCs cultured on collagen I and a naturally derived cardiac extracellular matrix.
Cyclic mechanical strain induced the strongest CPC alignment when cultured on fibronectin or collagen I.
This study demonstrates that culturing CPCs on fibronectin with 5% strain magnitude is optimal for their vascular endothelial growth factor A secretion, proliferation, connexin43 expression, and alignment.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
French, Kristin M.& Maxwell, Joshua T.& Bhutani, Srishti& Ghosh-Choudhary, Shohini& Fierro, Marcos J.& Johnson, Todd D.…[et al.]. 2016. Fibronectin and Cyclic Strain Improve Cardiac Progenitor Cell Regenerative Potential In Vitro. Stem Cells International،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1117255
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
French, Kristin M.…[et al.]. Fibronectin and Cyclic Strain Improve Cardiac Progenitor Cell Regenerative Potential In Vitro. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1117255
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
French, Kristin M.& Maxwell, Joshua T.& Bhutani, Srishti& Ghosh-Choudhary, Shohini& Fierro, Marcos J.& Johnson, Todd D.…[et al.]. Fibronectin and Cyclic Strain Improve Cardiac Progenitor Cell Regenerative Potential In Vitro. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1117255
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1117255
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر