Fibronectin and Cyclic Strain Improve Cardiac Progenitor Cell Regenerative Potential In Vitro
Joint Authors
French, Kristin M.
Maxwell, Joshua T.
Bhutani, Srishti
Ghosh-Choudhary, Shohini
Fierro, Marcos J.
Johnson, Todd D.
Christman, Karen L.
Taylor, W. Robert
Davis, Michael E.
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-08-16
Country of Publication
Egypt
No. of Pages
11
Abstract EN
Cardiac progenitor cells (CPCs) have rapidly advanced to clinical trials, yet little is known regarding their interaction with the microenvironment.
Signaling cues present in the microenvironment change with development and disease.
This work aims to assess the influence of two distinct signaling moieties on CPCs: cyclic biaxial strain and extracellular matrix.
We evaluate four endpoints for improving CPC therapy: paracrine signaling, proliferation, connexin43 expression, and alignment.
Vascular endothelial growth factor A (about 900 pg/mL) was secreted by CPCs cultured on fibronectin and collagen I.
The application of mechanical strain increased vascular endothelial growth factor A secretion 2–4-fold for CPCs cultured on poly-L-lysine, laminin, or a naturally derived cardiac extracellular matrix.
CPC proliferation was at least 25% higher on fibronectin than that on other matrices, especially for lower strain magnitudes.
At 5% strain, connexin43 expression was highest on fibronectin.
With increasing strain magnitude, connexin43 expression decreased by as much as 60% in CPCs cultured on collagen I and a naturally derived cardiac extracellular matrix.
Cyclic mechanical strain induced the strongest CPC alignment when cultured on fibronectin or collagen I.
This study demonstrates that culturing CPCs on fibronectin with 5% strain magnitude is optimal for their vascular endothelial growth factor A secretion, proliferation, connexin43 expression, and alignment.
American Psychological Association (APA)
French, Kristin M.& Maxwell, Joshua T.& Bhutani, Srishti& Ghosh-Choudhary, Shohini& Fierro, Marcos J.& Johnson, Todd D.…[et al.]. 2016. Fibronectin and Cyclic Strain Improve Cardiac Progenitor Cell Regenerative Potential In Vitro. Stem Cells International،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1117255
Modern Language Association (MLA)
French, Kristin M.…[et al.]. Fibronectin and Cyclic Strain Improve Cardiac Progenitor Cell Regenerative Potential In Vitro. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1117255
American Medical Association (AMA)
French, Kristin M.& Maxwell, Joshua T.& Bhutani, Srishti& Ghosh-Choudhary, Shohini& Fierro, Marcos J.& Johnson, Todd D.…[et al.]. Fibronectin and Cyclic Strain Improve Cardiac Progenitor Cell Regenerative Potential In Vitro. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1117255
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1117255