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Highly Synchronized Expression of Lineage-Specific Genes during In Vitro Hepatic Differentiation of Human Pluripotent Stem Cell Lines
المؤلفون المشاركون
Sartipy, Peter
Björquist, Petter
Edsbagge, Josefina
Synnergren, Jane
Ghosheh, Nidal
Küppers-Munther, Barbara
Van Giezen, Mariska
Asplund, Annika
Andersson, Tommy B.
Carén, Helena
Simonsson, Stina
Olsson, Björn
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-22، 22ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-02-01
دولة النشر
مصر
عدد الصفحات
22
الملخص EN
Human pluripotent stem cells- (hPSCs-) derived hepatocytes have the potential to replace many hepatic models in drug discovery and provide a cell source for regenerative medicine applications.
However, the generation of fully functional hPSC-derived hepatocytes is still a challenge.
Towards gaining better understanding of the differentiation and maturation process, we employed a standardized protocol to differentiate six hPSC lines into hepatocytes and investigated the synchronicity of the hPSC lines by applying RT-qPCR to assess the expression of lineage-specific genes (OCT4, NANOG, T, SOX17, CXCR4, CER1, HHEX, TBX3, PROX1, HNF6, AFP, HNF4a, KRT18, ALB, AAT, and CYP3A4) which serve as markers for different stages during liver development.
The data was evaluated using correlation and clustering analysis, demonstrating that the expression of these markers is highly synchronized and correlated well across all cell lines.
The analysis also revealed a distribution of the markers in groups reflecting the developmental stages of hepatocytes.
Functional analysis of the differentiated cells further confirmed their hepatic phenotype.
Taken together, these results demonstrate, on the molecular level, the highly synchronized differentiation pattern across multiple hPSC lines.
Moreover, this study provides additional understanding for future efforts to improve the functionality of hPSC-derived hepatocytes and thereby increase the value of related models.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ghosheh, Nidal& Olsson, Björn& Edsbagge, Josefina& Küppers-Munther, Barbara& Van Giezen, Mariska& Asplund, Annika…[et al.]. 2016. Highly Synchronized Expression of Lineage-Specific Genes during In Vitro Hepatic Differentiation of Human Pluripotent Stem Cell Lines. Stem Cells International،Vol. 2016, no. 2016, pp.1-22.
https://search.emarefa.net/detail/BIM-1117302
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ghosheh, Nidal…[et al.]. Highly Synchronized Expression of Lineage-Specific Genes during In Vitro Hepatic Differentiation of Human Pluripotent Stem Cell Lines. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-22.
https://search.emarefa.net/detail/BIM-1117302
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ghosheh, Nidal& Olsson, Björn& Edsbagge, Josefina& Küppers-Munther, Barbara& Van Giezen, Mariska& Asplund, Annika…[et al.]. Highly Synchronized Expression of Lineage-Specific Genes during In Vitro Hepatic Differentiation of Human Pluripotent Stem Cell Lines. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-22.
https://search.emarefa.net/detail/BIM-1117302
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1117302
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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