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Highly Synchronized Expression of Lineage-Specific Genes during In Vitro Hepatic Differentiation of Human Pluripotent Stem Cell Lines
Joint Authors
Sartipy, Peter
Björquist, Petter
Edsbagge, Josefina
Synnergren, Jane
Ghosheh, Nidal
Küppers-Munther, Barbara
Van Giezen, Mariska
Asplund, Annika
Andersson, Tommy B.
Carén, Helena
Simonsson, Stina
Olsson, Björn
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-22, 22 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-02-01
Country of Publication
Egypt
No. of Pages
22
Abstract EN
Human pluripotent stem cells- (hPSCs-) derived hepatocytes have the potential to replace many hepatic models in drug discovery and provide a cell source for regenerative medicine applications.
However, the generation of fully functional hPSC-derived hepatocytes is still a challenge.
Towards gaining better understanding of the differentiation and maturation process, we employed a standardized protocol to differentiate six hPSC lines into hepatocytes and investigated the synchronicity of the hPSC lines by applying RT-qPCR to assess the expression of lineage-specific genes (OCT4, NANOG, T, SOX17, CXCR4, CER1, HHEX, TBX3, PROX1, HNF6, AFP, HNF4a, KRT18, ALB, AAT, and CYP3A4) which serve as markers for different stages during liver development.
The data was evaluated using correlation and clustering analysis, demonstrating that the expression of these markers is highly synchronized and correlated well across all cell lines.
The analysis also revealed a distribution of the markers in groups reflecting the developmental stages of hepatocytes.
Functional analysis of the differentiated cells further confirmed their hepatic phenotype.
Taken together, these results demonstrate, on the molecular level, the highly synchronized differentiation pattern across multiple hPSC lines.
Moreover, this study provides additional understanding for future efforts to improve the functionality of hPSC-derived hepatocytes and thereby increase the value of related models.
American Psychological Association (APA)
Ghosheh, Nidal& Olsson, Björn& Edsbagge, Josefina& Küppers-Munther, Barbara& Van Giezen, Mariska& Asplund, Annika…[et al.]. 2016. Highly Synchronized Expression of Lineage-Specific Genes during In Vitro Hepatic Differentiation of Human Pluripotent Stem Cell Lines. Stem Cells International،Vol. 2016, no. 2016, pp.1-22.
https://search.emarefa.net/detail/BIM-1117302
Modern Language Association (MLA)
Ghosheh, Nidal…[et al.]. Highly Synchronized Expression of Lineage-Specific Genes during In Vitro Hepatic Differentiation of Human Pluripotent Stem Cell Lines. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-22.
https://search.emarefa.net/detail/BIM-1117302
American Medical Association (AMA)
Ghosheh, Nidal& Olsson, Björn& Edsbagge, Josefina& Küppers-Munther, Barbara& Van Giezen, Mariska& Asplund, Annika…[et al.]. Highly Synchronized Expression of Lineage-Specific Genes during In Vitro Hepatic Differentiation of Human Pluripotent Stem Cell Lines. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-22.
https://search.emarefa.net/detail/BIM-1117302
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1117302