A Controlled Release Codelivery System of MSCs Encapsulated in DextranGelatin Hydrogel with TGF-β3-Loaded Nanoparticles for Nucleus Pulposus Regeneration
المؤلفون المشاركون
Mo, Xiumei
Gan, Yibo
Zhou, Qiang
Zhang, Chengmin
Xu, Yuan
Li, Pei
Li, Sukai
Wang, Liyuan
Zhao, Chen
Ouyang, Bin
Tu, Bing
Luo, Lei
Luo, Xiangdong
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-14، 14ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-09-27
دولة النشر
مصر
عدد الصفحات
14
الملخص EN
Mesenchymal stem cell- (MSC-) based therapy is regarded as a potential tissue engineering strategy to achieve nucleus pulposus (NP) regeneration for the treatment of intervertebral disc degeneration (IDD).
However, it is still a challenge to induce MSC differentiation in NP-like cells when MSCs are implanted into the NP.
The purpose of this study was to construct poly(D,L-lactide-co-glycolide) (PLGA) nanoparticles as carriers for TGF-β3 controlled release and establish a codelivery system of a dextran/gelatin hydrogel with the nanoparticles for long-term processing of discogenesis differentiation.
TGF-β3-loaded PLGA nanoparticles were prepared by the double-emulsion solvent evaporation method and seeded uniformly into the hydrogel.
Morphological observations, an assessment of the release kinetics of TGF-β3, a cytotoxic assay, a cell proliferation test, a biochemical content assay, qRT-PCR, and immunohistological analyses of the codelivery system were conducted in the study.
The results showed that the TGF-β3-loaded nanoparticles could release TGF-β3 gradually.
The codelivery system exhibited favorable cytocompatibility, and the TGF-β3 that was released could induce MSCs to NP-like cells while promoting ECM-related biosynthesis.
These results suggest this codelivery system may be employed as a promising carrier for discogenesis of MSCs in situ.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Gan, Yibo& Li, Sukai& Li, Pei& Xu, Yuan& Wang, Liyuan& Zhao, Chen…[et al.]. 2016. A Controlled Release Codelivery System of MSCs Encapsulated in DextranGelatin Hydrogel with TGF-β3-Loaded Nanoparticles for Nucleus Pulposus Regeneration. Stem Cells International،Vol. 2016, no. 2016, pp.1-14.
https://search.emarefa.net/detail/BIM-1117356
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Gan, Yibo…[et al.]. A Controlled Release Codelivery System of MSCs Encapsulated in DextranGelatin Hydrogel with TGF-β3-Loaded Nanoparticles for Nucleus Pulposus Regeneration. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-14.
https://search.emarefa.net/detail/BIM-1117356
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Gan, Yibo& Li, Sukai& Li, Pei& Xu, Yuan& Wang, Liyuan& Zhao, Chen…[et al.]. A Controlled Release Codelivery System of MSCs Encapsulated in DextranGelatin Hydrogel with TGF-β3-Loaded Nanoparticles for Nucleus Pulposus Regeneration. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-14.
https://search.emarefa.net/detail/BIM-1117356
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1117356
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر