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A Controlled Release Codelivery System of MSCs Encapsulated in DextranGelatin Hydrogel with TGF-β3-Loaded Nanoparticles for Nucleus Pulposus Regeneration
Joint Authors
Mo, Xiumei
Gan, Yibo
Zhou, Qiang
Zhang, Chengmin
Xu, Yuan
Li, Pei
Li, Sukai
Wang, Liyuan
Zhao, Chen
Ouyang, Bin
Tu, Bing
Luo, Lei
Luo, Xiangdong
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-09-27
Country of Publication
Egypt
No. of Pages
14
Abstract EN
Mesenchymal stem cell- (MSC-) based therapy is regarded as a potential tissue engineering strategy to achieve nucleus pulposus (NP) regeneration for the treatment of intervertebral disc degeneration (IDD).
However, it is still a challenge to induce MSC differentiation in NP-like cells when MSCs are implanted into the NP.
The purpose of this study was to construct poly(D,L-lactide-co-glycolide) (PLGA) nanoparticles as carriers for TGF-β3 controlled release and establish a codelivery system of a dextran/gelatin hydrogel with the nanoparticles for long-term processing of discogenesis differentiation.
TGF-β3-loaded PLGA nanoparticles were prepared by the double-emulsion solvent evaporation method and seeded uniformly into the hydrogel.
Morphological observations, an assessment of the release kinetics of TGF-β3, a cytotoxic assay, a cell proliferation test, a biochemical content assay, qRT-PCR, and immunohistological analyses of the codelivery system were conducted in the study.
The results showed that the TGF-β3-loaded nanoparticles could release TGF-β3 gradually.
The codelivery system exhibited favorable cytocompatibility, and the TGF-β3 that was released could induce MSCs to NP-like cells while promoting ECM-related biosynthesis.
These results suggest this codelivery system may be employed as a promising carrier for discogenesis of MSCs in situ.
American Psychological Association (APA)
Gan, Yibo& Li, Sukai& Li, Pei& Xu, Yuan& Wang, Liyuan& Zhao, Chen…[et al.]. 2016. A Controlled Release Codelivery System of MSCs Encapsulated in DextranGelatin Hydrogel with TGF-β3-Loaded Nanoparticles for Nucleus Pulposus Regeneration. Stem Cells International،Vol. 2016, no. 2016, pp.1-14.
https://search.emarefa.net/detail/BIM-1117356
Modern Language Association (MLA)
Gan, Yibo…[et al.]. A Controlled Release Codelivery System of MSCs Encapsulated in DextranGelatin Hydrogel with TGF-β3-Loaded Nanoparticles for Nucleus Pulposus Regeneration. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-14.
https://search.emarefa.net/detail/BIM-1117356
American Medical Association (AMA)
Gan, Yibo& Li, Sukai& Li, Pei& Xu, Yuan& Wang, Liyuan& Zhao, Chen…[et al.]. A Controlled Release Codelivery System of MSCs Encapsulated in DextranGelatin Hydrogel with TGF-β3-Loaded Nanoparticles for Nucleus Pulposus Regeneration. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-14.
https://search.emarefa.net/detail/BIM-1117356
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1117356