Effect of Fibroblast Growth Factor 2 on Equine Synovial Fluid Chondroprogenitor Expansion and Chondrogenesis
المؤلفون المشاركون
Bianchessi, Marta
Chen, Yuwen
Durgam, Sushmitha
Stewart, Matthew
Pondenis, Holly C.
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-12-29
دولة النشر
مصر
عدد الصفحات
11
الملخص EN
Mesenchymal stem cells have been identified in the synovial fluid of several species.
This study was conducted to characterize chondroprogenitor (CP) cells in equine synovial fluid (SF) and to determine the effect of fibroblast growth factor 2 (FGF-2) on SF-CP monolayer proliferation and subsequent chondrogenesis.
We hypothesized that FGF-2 would stimulate SF-CP proliferation and postexpansion chondrogenesis.
SF aspirates were collected from adult equine joints.
Colony-forming unit (CFU) assays were performed during primary cultures.
At first passage, SF-cells were seeded at low density, with or without FGF-2.
Following monolayer expansion and serial immunophenotyping, cells were transferred to chondrogenic pellet cultures.
Pellets were analyzed for chondrogenic mRNA expression and cartilage matrix secretion.
There was a mean of 59.2 CFU/mL of SF.
FGF-2 increased the number of population doublings during two monolayer passages and halved the population doubling times.
FGF-2 did not alter the immunophenotype of SF-CPs during monolayer expansion, nor did FGF-2 compromise chondrogenesis.
Hypertrophic phenotypic markers were not expressed in control or FGF-2 groups.
FGF-2 did prevent the development of a “fibroblastic” cell layer around pellet periphery.
FGF-2 significantly accelerates in vitro SF-CP expansion, the major hurdle to clinical application of this cell population, without detrimentally affecting subsequent chondrogenic capacity.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Bianchessi, Marta& Chen, Yuwen& Durgam, Sushmitha& Pondenis, Holly C.& Stewart, Matthew. 2015. Effect of Fibroblast Growth Factor 2 on Equine Synovial Fluid Chondroprogenitor Expansion and Chondrogenesis. Stem Cells International،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1117409
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Bianchessi, Marta…[et al.]. Effect of Fibroblast Growth Factor 2 on Equine Synovial Fluid Chondroprogenitor Expansion and Chondrogenesis. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1117409
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Bianchessi, Marta& Chen, Yuwen& Durgam, Sushmitha& Pondenis, Holly C.& Stewart, Matthew. Effect of Fibroblast Growth Factor 2 on Equine Synovial Fluid Chondroprogenitor Expansion and Chondrogenesis. Stem Cells International. 2015. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1117409
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1117409
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر