Effect of Fibroblast Growth Factor 2 on Equine Synovial Fluid Chondroprogenitor Expansion and Chondrogenesis
Joint Authors
Bianchessi, Marta
Chen, Yuwen
Durgam, Sushmitha
Stewart, Matthew
Pondenis, Holly C.
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-12-29
Country of Publication
Egypt
No. of Pages
11
Abstract EN
Mesenchymal stem cells have been identified in the synovial fluid of several species.
This study was conducted to characterize chondroprogenitor (CP) cells in equine synovial fluid (SF) and to determine the effect of fibroblast growth factor 2 (FGF-2) on SF-CP monolayer proliferation and subsequent chondrogenesis.
We hypothesized that FGF-2 would stimulate SF-CP proliferation and postexpansion chondrogenesis.
SF aspirates were collected from adult equine joints.
Colony-forming unit (CFU) assays were performed during primary cultures.
At first passage, SF-cells were seeded at low density, with or without FGF-2.
Following monolayer expansion and serial immunophenotyping, cells were transferred to chondrogenic pellet cultures.
Pellets were analyzed for chondrogenic mRNA expression and cartilage matrix secretion.
There was a mean of 59.2 CFU/mL of SF.
FGF-2 increased the number of population doublings during two monolayer passages and halved the population doubling times.
FGF-2 did not alter the immunophenotype of SF-CPs during monolayer expansion, nor did FGF-2 compromise chondrogenesis.
Hypertrophic phenotypic markers were not expressed in control or FGF-2 groups.
FGF-2 did prevent the development of a “fibroblastic” cell layer around pellet periphery.
FGF-2 significantly accelerates in vitro SF-CP expansion, the major hurdle to clinical application of this cell population, without detrimentally affecting subsequent chondrogenic capacity.
American Psychological Association (APA)
Bianchessi, Marta& Chen, Yuwen& Durgam, Sushmitha& Pondenis, Holly C.& Stewart, Matthew. 2015. Effect of Fibroblast Growth Factor 2 on Equine Synovial Fluid Chondroprogenitor Expansion and Chondrogenesis. Stem Cells International،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1117409
Modern Language Association (MLA)
Bianchessi, Marta…[et al.]. Effect of Fibroblast Growth Factor 2 on Equine Synovial Fluid Chondroprogenitor Expansion and Chondrogenesis. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1117409
American Medical Association (AMA)
Bianchessi, Marta& Chen, Yuwen& Durgam, Sushmitha& Pondenis, Holly C.& Stewart, Matthew. Effect of Fibroblast Growth Factor 2 on Equine Synovial Fluid Chondroprogenitor Expansion and Chondrogenesis. Stem Cells International. 2015. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1117409
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1117409