Sickled Erythrocytes Reversal and Membrane Stabilizing Compounds in Telfairia occidentalis
المؤلفون المشاركون
Atabo, Samuel
Umar, Ismaila Alhaji
James, Dorcas Bolanle
Mamman, Aisha Indo
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-06-28
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Background and Purpose.
Traditional management of sickle cell disease (SCD) is ubiquitous in Africa.
In south-eastern Nigeria, Telfairia occidentalis (T.
occidentalis) is strongly recommended for consumption by SCD patients, owing to its presumed therapeutic effect.
This study investigates the antisickling and membrane regenerative potentials of T.
occidentalis in sickled erythrocytes.
Experimental Approach.
Sickled erythrocytes obtained from SCD patients were treated with sodium metabisulphite (2%) to induce further sickling.
Heat and hypotonic-induced lyses of red blood cells’ membranes were also carried out.
The RBCs were treated with varying concentration (10.0, 1.0, and 0.1 mg mL−1 and 0.5, 1.0, 1.5, 2.0, and 2.5 mg mL−1, resp.) of T.
occidentalis extracts as treatment regimen for in vitro antisickling and membrane stabilizing assays.
Extract with peak activity was purified and reused in antisickling assay.
Key Results.
The antisickling activity of aqueous and methanolic extracts of leaves, seeds, and stem of Telfairia occidentalis at 10.0, 1.0, and 0.1 mg mL−1 revealed that the aqueous leaves extract (10 mg mL−1) exhibited the highest antisickling activity (64.03%) which was significantly ( p < 0.05 ) higher than that of the stem (47.30%) and seeds (37.50%).
Partially purified fractions recorded improved antisickling effect (peak activity of 70%).
Characterization (using GC-MS) of the most active fraction revealed some bioactive compounds.
In the membrane stabilizing assay, methanolic and aqueous stem extracts of T.
occidentalis showed the highest effect of 71.85% and 61.29%, respectively.
Conclusions and Implications.
The results provide scientific evidence for ethnopharmacological use of T.
occidentalis in the management of SCD.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Atabo, Samuel& Umar, Ismaila Alhaji& James, Dorcas Bolanle& Mamman, Aisha Indo. 2016. Sickled Erythrocytes Reversal and Membrane Stabilizing Compounds in Telfairia occidentalis. Scientifica،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1117548
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Atabo, Samuel…[et al.]. Sickled Erythrocytes Reversal and Membrane Stabilizing Compounds in Telfairia occidentalis. Scientifica No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1117548
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Atabo, Samuel& Umar, Ismaila Alhaji& James, Dorcas Bolanle& Mamman, Aisha Indo. Sickled Erythrocytes Reversal and Membrane Stabilizing Compounds in Telfairia occidentalis. Scientifica. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1117548
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1117548
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر