Sickled Erythrocytes Reversal and Membrane Stabilizing Compounds in Telfairia occidentalis
Joint Authors
Atabo, Samuel
Umar, Ismaila Alhaji
James, Dorcas Bolanle
Mamman, Aisha Indo
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-06-28
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Background and Purpose.
Traditional management of sickle cell disease (SCD) is ubiquitous in Africa.
In south-eastern Nigeria, Telfairia occidentalis (T.
occidentalis) is strongly recommended for consumption by SCD patients, owing to its presumed therapeutic effect.
This study investigates the antisickling and membrane regenerative potentials of T.
occidentalis in sickled erythrocytes.
Experimental Approach.
Sickled erythrocytes obtained from SCD patients were treated with sodium metabisulphite (2%) to induce further sickling.
Heat and hypotonic-induced lyses of red blood cells’ membranes were also carried out.
The RBCs were treated with varying concentration (10.0, 1.0, and 0.1 mg mL−1 and 0.5, 1.0, 1.5, 2.0, and 2.5 mg mL−1, resp.) of T.
occidentalis extracts as treatment regimen for in vitro antisickling and membrane stabilizing assays.
Extract with peak activity was purified and reused in antisickling assay.
Key Results.
The antisickling activity of aqueous and methanolic extracts of leaves, seeds, and stem of Telfairia occidentalis at 10.0, 1.0, and 0.1 mg mL−1 revealed that the aqueous leaves extract (10 mg mL−1) exhibited the highest antisickling activity (64.03%) which was significantly ( p < 0.05 ) higher than that of the stem (47.30%) and seeds (37.50%).
Partially purified fractions recorded improved antisickling effect (peak activity of 70%).
Characterization (using GC-MS) of the most active fraction revealed some bioactive compounds.
In the membrane stabilizing assay, methanolic and aqueous stem extracts of T.
occidentalis showed the highest effect of 71.85% and 61.29%, respectively.
Conclusions and Implications.
The results provide scientific evidence for ethnopharmacological use of T.
occidentalis in the management of SCD.
American Psychological Association (APA)
Atabo, Samuel& Umar, Ismaila Alhaji& James, Dorcas Bolanle& Mamman, Aisha Indo. 2016. Sickled Erythrocytes Reversal and Membrane Stabilizing Compounds in Telfairia occidentalis. Scientifica،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1117548
Modern Language Association (MLA)
Atabo, Samuel…[et al.]. Sickled Erythrocytes Reversal and Membrane Stabilizing Compounds in Telfairia occidentalis. Scientifica No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1117548
American Medical Association (AMA)
Atabo, Samuel& Umar, Ismaila Alhaji& James, Dorcas Bolanle& Mamman, Aisha Indo. Sickled Erythrocytes Reversal and Membrane Stabilizing Compounds in Telfairia occidentalis. Scientifica. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1117548
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1117548