Conditioned Medium from Adipose-Derived Stem Cell Inhibits Jurkat Cell Proliferation through TGF-β1 and p38MAPK Pathway
المؤلفون المشاركون
Wang, Xiuxia
Wang, Yinmin
Zhou, Xianyu
Liu, Fei
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-12-19
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
Background.
Since the first report on the immunomodulatory and immunosuppressive properties of Adipose-Derived Stem Cells (ADSCs), many studies have elucidated the underlying molecular mechanism of their suppressive activity on mixed lymphocyte reaction (MLR).
However, a gap exists in our understanding of the molecular mechanism of ADSC-conditioned medium (ADSC-CM) on MLR.
Methods.
ADSCs were isolated from Human Adipose Tissues, and Enzyme-linked Immunosorbent Assay (ELISA) was used to identify the concentration of transforming growth factor β1 (TGF-β1) in ADSC-CM.
The transcript abundance of TGF-β1, as well as that of insulin-like growth factor binding protein 3 (IGF-BP3), was evaluated using qRT-PCR on Jurkat cells cultured in ADSC-CM for 24 hours.
The proliferation of the Jurkat cells was assessed using cell cycle assay.
Western blotting was performed to identify potential signaling molecules involved in the ADSC-CM-induced inhibition of Jurkat cell proliferation.
Results.
The findings confirm that the isolated ADSCs demonstrate classic ADSC characteristics.
The level of TGF-β1 was found to be low in ADSC-CM, as assessed by ELISA.
Jurkat cells grown in ADSC-CM show reduced gene expression of TGF-β1 and IGF-BP3 compared with that of the control group.
Furthermore, western blotting of ADSC-CM grown Jurkat cells that were blocked at the G0/G1 stage indicates that ADSC-CM decreases the protein expression of pP38 in a dose-dependent manner.
Conclusion.
ADSC-CM can inhibit Jurkat cell proliferation through the TGF-β1-p38 signaling pathway.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wang, Xiuxia& Wang, Yinmin& Zhou, Xianyu& Liu, Fei. 2019. Conditioned Medium from Adipose-Derived Stem Cell Inhibits Jurkat Cell Proliferation through TGF-β1 and p38MAPK Pathway. Analytical Cellular Pathology،Vol. 2019, no. 2019, pp.1-6.
https://search.emarefa.net/detail/BIM-1117792
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wang, Xiuxia…[et al.]. Conditioned Medium from Adipose-Derived Stem Cell Inhibits Jurkat Cell Proliferation through TGF-β1 and p38MAPK Pathway. Analytical Cellular Pathology No. 2019 (2019), pp.1-6.
https://search.emarefa.net/detail/BIM-1117792
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wang, Xiuxia& Wang, Yinmin& Zhou, Xianyu& Liu, Fei. Conditioned Medium from Adipose-Derived Stem Cell Inhibits Jurkat Cell Proliferation through TGF-β1 and p38MAPK Pathway. Analytical Cellular Pathology. 2019. Vol. 2019, no. 2019, pp.1-6.
https://search.emarefa.net/detail/BIM-1117792
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1117792
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر