Conditioned Medium from Adipose-Derived Stem Cell Inhibits Jurkat Cell Proliferation through TGF-β1 and p38MAPK Pathway
Joint Authors
Wang, Xiuxia
Wang, Yinmin
Zhou, Xianyu
Liu, Fei
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-12-19
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Background.
Since the first report on the immunomodulatory and immunosuppressive properties of Adipose-Derived Stem Cells (ADSCs), many studies have elucidated the underlying molecular mechanism of their suppressive activity on mixed lymphocyte reaction (MLR).
However, a gap exists in our understanding of the molecular mechanism of ADSC-conditioned medium (ADSC-CM) on MLR.
Methods.
ADSCs were isolated from Human Adipose Tissues, and Enzyme-linked Immunosorbent Assay (ELISA) was used to identify the concentration of transforming growth factor β1 (TGF-β1) in ADSC-CM.
The transcript abundance of TGF-β1, as well as that of insulin-like growth factor binding protein 3 (IGF-BP3), was evaluated using qRT-PCR on Jurkat cells cultured in ADSC-CM for 24 hours.
The proliferation of the Jurkat cells was assessed using cell cycle assay.
Western blotting was performed to identify potential signaling molecules involved in the ADSC-CM-induced inhibition of Jurkat cell proliferation.
Results.
The findings confirm that the isolated ADSCs demonstrate classic ADSC characteristics.
The level of TGF-β1 was found to be low in ADSC-CM, as assessed by ELISA.
Jurkat cells grown in ADSC-CM show reduced gene expression of TGF-β1 and IGF-BP3 compared with that of the control group.
Furthermore, western blotting of ADSC-CM grown Jurkat cells that were blocked at the G0/G1 stage indicates that ADSC-CM decreases the protein expression of pP38 in a dose-dependent manner.
Conclusion.
ADSC-CM can inhibit Jurkat cell proliferation through the TGF-β1-p38 signaling pathway.
American Psychological Association (APA)
Wang, Xiuxia& Wang, Yinmin& Zhou, Xianyu& Liu, Fei. 2019. Conditioned Medium from Adipose-Derived Stem Cell Inhibits Jurkat Cell Proliferation through TGF-β1 and p38MAPK Pathway. Analytical Cellular Pathology،Vol. 2019, no. 2019, pp.1-6.
https://search.emarefa.net/detail/BIM-1117792
Modern Language Association (MLA)
Wang, Xiuxia…[et al.]. Conditioned Medium from Adipose-Derived Stem Cell Inhibits Jurkat Cell Proliferation through TGF-β1 and p38MAPK Pathway. Analytical Cellular Pathology No. 2019 (2019), pp.1-6.
https://search.emarefa.net/detail/BIM-1117792
American Medical Association (AMA)
Wang, Xiuxia& Wang, Yinmin& Zhou, Xianyu& Liu, Fei. Conditioned Medium from Adipose-Derived Stem Cell Inhibits Jurkat Cell Proliferation through TGF-β1 and p38MAPK Pathway. Analytical Cellular Pathology. 2019. Vol. 2019, no. 2019, pp.1-6.
https://search.emarefa.net/detail/BIM-1117792
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1117792