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Identification and Verification of the Main Differentially Expressed Proteins in Gastric Cancer via iTRAQ Combined with Liquid Chromatography-Mass Spectrometry
المؤلفون المشاركون
Gao, Zhihua
Wang, Jiabao
Bai, Yuru
Bao, Jun
Dai, Erqing
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-14، 14ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-12-01
دولة النشر
مصر
عدد الصفحات
14
التخصصات الرئيسية
الملخص EN
Background.
To find the potential intersections between the differentially expressed proteins and abnormally expressed genes in gastric cancer (GC) patients.
Methods.
Gastric cancer tissue and adjacent normal mucosa tissue were used for iTRAQ analysis.
Gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, and protein-protein interaction (PPI) analysis were used to evaluate gene function.
Western blotting and immunohistochemistry (IHC) were applied to verify the protein expression.
Results.
A total of 2770 proteins were identified, of which 147 proteins were upregulated and 159 proteins were downregulated.
GO analysis revealed that the differentially expressed genes were mainly enriched for the terms “cellular process,” “binding,” and “cell.” The results of the KEGG analysis showed that the most abundantly enriched proteins were involved in the “focal adhesion” pathway.
The results of the PPI analysis showed that VCAM1 was located at the center of the PPI network.
Western blotting and IHC analysis demonstrated that VCAM1, FLNA, VASP, CAV1, PICK1, and COL4A2 were differentially expressed in GC and adjacent normal tissues, which was consistent with the results of the iTRAQ analysis.
Conclusion.
In conclusion, 6 highly differentially expressed proteins were identified as novel differentially expressed proteins in human GC.
This exploratory research may provide useful information for the treatment of gastric cancer in the clinic.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Gao, Zhihua& Wang, Jiabao& Bai, Yuru& Bao, Jun& Dai, Erqing. 2019. Identification and Verification of the Main Differentially Expressed Proteins in Gastric Cancer via iTRAQ Combined with Liquid Chromatography-Mass Spectrometry. Analytical Cellular Pathology،Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1117856
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Gao, Zhihua…[et al.]. Identification and Verification of the Main Differentially Expressed Proteins in Gastric Cancer via iTRAQ Combined with Liquid Chromatography-Mass Spectrometry. Analytical Cellular Pathology No. 2019 (2019), pp.1-14.
https://search.emarefa.net/detail/BIM-1117856
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Gao, Zhihua& Wang, Jiabao& Bai, Yuru& Bao, Jun& Dai, Erqing. Identification and Verification of the Main Differentially Expressed Proteins in Gastric Cancer via iTRAQ Combined with Liquid Chromatography-Mass Spectrometry. Analytical Cellular Pathology. 2019. Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1117856
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1117856
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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