Identification and Verification of the Main Differentially Expressed Proteins in Gastric Cancer via iTRAQ Combined with Liquid Chromatography-Mass Spectrometry

Joint Authors

Gao, Zhihua
Wang, Jiabao
Bai, Yuru
Bao, Jun
Dai, Erqing

Source

Analytical Cellular Pathology

Issue

Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-14, 14 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2019-12-01

Country of Publication

Egypt

No. of Pages

14

Main Subjects

Diseases
Medicine

Abstract EN

Background.

To find the potential intersections between the differentially expressed proteins and abnormally expressed genes in gastric cancer (GC) patients.

Methods.

Gastric cancer tissue and adjacent normal mucosa tissue were used for iTRAQ analysis.

Gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, and protein-protein interaction (PPI) analysis were used to evaluate gene function.

Western blotting and immunohistochemistry (IHC) were applied to verify the protein expression.

Results.

A total of 2770 proteins were identified, of which 147 proteins were upregulated and 159 proteins were downregulated.

GO analysis revealed that the differentially expressed genes were mainly enriched for the terms “cellular process,” “binding,” and “cell.” The results of the KEGG analysis showed that the most abundantly enriched proteins were involved in the “focal adhesion” pathway.

The results of the PPI analysis showed that VCAM1 was located at the center of the PPI network.

Western blotting and IHC analysis demonstrated that VCAM1, FLNA, VASP, CAV1, PICK1, and COL4A2 were differentially expressed in GC and adjacent normal tissues, which was consistent with the results of the iTRAQ analysis.

Conclusion.

In conclusion, 6 highly differentially expressed proteins were identified as novel differentially expressed proteins in human GC.

This exploratory research may provide useful information for the treatment of gastric cancer in the clinic.

American Psychological Association (APA)

Gao, Zhihua& Wang, Jiabao& Bai, Yuru& Bao, Jun& Dai, Erqing. 2019. Identification and Verification of the Main Differentially Expressed Proteins in Gastric Cancer via iTRAQ Combined with Liquid Chromatography-Mass Spectrometry. Analytical Cellular Pathology،Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1117856

Modern Language Association (MLA)

Gao, Zhihua…[et al.]. Identification and Verification of the Main Differentially Expressed Proteins in Gastric Cancer via iTRAQ Combined with Liquid Chromatography-Mass Spectrometry. Analytical Cellular Pathology No. 2019 (2019), pp.1-14.
https://search.emarefa.net/detail/BIM-1117856

American Medical Association (AMA)

Gao, Zhihua& Wang, Jiabao& Bai, Yuru& Bao, Jun& Dai, Erqing. Identification and Verification of the Main Differentially Expressed Proteins in Gastric Cancer via iTRAQ Combined with Liquid Chromatography-Mass Spectrometry. Analytical Cellular Pathology. 2019. Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1117856

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1117856