In Vitro Cytotoxicity of Calcium Silicate-Based Endodontic Cement as Root-End Filling Materials

المؤلفون المشاركون

Küçükkaya, Selen
Görduysus, Mehmet Ömer
Zeybek, Naciye Dilara
Müftüoğlu, Sevda Fatma

المصدر

Scientifica

العدد

المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-5، 5ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2016-01-21

دولة النشر

مصر

عدد الصفحات

5

التخصصات الرئيسية

الأمراض

الملخص EN

The aim of this study was to evaluate the cytotoxicity of three types of calcium silicate-based endodontic cement after different incubation periods with human periodontal ligament fibroblasts.

Human periodontal ligament fibroblasts were cultured from extracted third molars and seeded in 96-well plates.

MTA, calcium enriched mixture (CEM) cement, and Biodentine were prepared and added to culture insert plates which were immediately placed into 96-well plates containing cultured cells.

After incubation periods of 24, 48, and 72 hours, cell viability was determined with WST-1 assay.

Data were analysed statistically by ANOVA with repeated measures and Bonferroni tests.

There was no significant difference in cell viability amongst the test materials after each incubation period ( P > 0.05 ).

MTA and CEM presented more than 90% cell viability after 24 and 48 hours of incubation and showed statistically significant decrease in cell viability after 72 hours of incubation ( P < 0.05 ).

Biodentine showed significantly less cell viability (73%) after 24 hours of incubation, whereas more than 90% cell viability was seen after 48 and 72 hours of incubation ( P < 0.05 ).

Despite the significant changes in cell viability over time, materials presented similar cytotoxicity profile.

Biodentine and CEM can be considered as alternative materials for root-end surgery procedures.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Küçükkaya, Selen& Görduysus, Mehmet Ömer& Zeybek, Naciye Dilara& Müftüoğlu, Sevda Fatma. 2016. In Vitro Cytotoxicity of Calcium Silicate-Based Endodontic Cement as Root-End Filling Materials. Scientifica،Vol. 2016, no. 2016, pp.1-5.
https://search.emarefa.net/detail/BIM-1117977

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Küçükkaya, Selen…[et al.]. In Vitro Cytotoxicity of Calcium Silicate-Based Endodontic Cement as Root-End Filling Materials. Scientifica No. 2016 (2016), pp.1-5.
https://search.emarefa.net/detail/BIM-1117977

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Küçükkaya, Selen& Görduysus, Mehmet Ömer& Zeybek, Naciye Dilara& Müftüoğlu, Sevda Fatma. In Vitro Cytotoxicity of Calcium Silicate-Based Endodontic Cement as Root-End Filling Materials. Scientifica. 2016. Vol. 2016, no. 2016, pp.1-5.
https://search.emarefa.net/detail/BIM-1117977

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1117977