In Vitro Cytotoxicity of Calcium Silicate-Based Endodontic Cement as Root-End Filling Materials

Abstract EN

The aim of this study was to evaluate the cytotoxicity of three types of calcium silicate-based endodontic cement after different incubation periods with human periodontal ligament fibroblasts.

Human periodontal ligament fibroblasts were cultured from extracted third molars and seeded in 96-well plates.

MTA, calcium enriched mixture (CEM) cement, and Biodentine were prepared and added to culture insert plates which were immediately placed into 96-well plates containing cultured cells.

After incubation periods of 24, 48, and 72 hours, cell viability was determined with WST-1 assay.

Data were analysed statistically by ANOVA with repeated measures and Bonferroni tests.

There was no significant difference in cell viability amongst the test materials after each incubation period ( P > 0.05 ).

MTA and CEM presented more than 90% cell viability after 24 and 48 hours of incubation and showed statistically significant decrease in cell viability after 72 hours of incubation ( P < 0.05 ).

Biodentine showed significantly less cell viability (73%) after 24 hours of incubation, whereas more than 90% cell viability was seen after 48 and 72 hours of incubation ( P < 0.05 ).

Despite the significant changes in cell viability over time, materials presented similar cytotoxicity profile.

Biodentine and CEM can be considered as alternative materials for root-end surgery procedures.