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Bionic Silk Fibroin Film Induces Morphological Changes and Differentiation of Tendon StemProgenitor Cells
المؤلفون المشاركون
Lai, Fan
Lu, Kang
Chen, Xiaodie
Tang, Hong
Zhou, Mei
He, Gang
Bian, Xuting
Guo, Yupeng
Yang, Mingyu
Lu, Zhisong
Tang, Kanglai
Liu, Juan
المصدر
Applied Bionics and Biomechanics
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-12-02
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Purpose.
Tendon injuries are common musculoskeletal system disorders, but the ability for tendon regeneration is limited.
Silk fibroin (SF) film may be suitable for tendon regeneration due to its excellent biocompatibility and physical properties.
This study is aimed at evaluating the application value of bionic SF film in tendon regeneration.
Methods.
Tendon stem/progenitor cells (TSPCs) were isolated from rat Achilles tendon and characterized based on their surface marker expression and multilineage differentiation potential.
SF films with smooth or bionic microstructure surfaces (5, 10, 15, 20 μm) were prepared.
The morphology and mechanical properties of natural tendons and SF films were characterized.
TSPCs were used as the seed cells, and the cell viability and cell adhesion morphology were analyzed.
The tendongenesis-related gene expression of TSPCs was also evaluated using quantitative polymerase chain reaction.
Results.
Compared to the native tendon, only the 10, 15, and 20 μm SF film groups had comparable maximum loading and ultimate stress, with the exception of the breaking elongation rate.
The 10 μm SF film group had the highest percentage of oriented cells and the most significant changes in cell morphology.
The most significant upregulations in the expression of COL1A1, TNC, TNMD, and SCX were also observed in the 10 μm SF film group.
Conclusion.
SF film with a bionic microstructure can serve as a tissue engineering scaffold and provide biophysical cues for the use of TSPCs to achieve proper cellular adherence arrangement and morphology as well as promote the tenogenic differentiation of TSPCs, making it a valuable customizable biomaterial for future applications in tendon repair.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Lu, Kang& Chen, Xiaodie& Tang, Hong& Zhou, Mei& He, Gang& Liu, Juan…[et al.]. 2020. Bionic Silk Fibroin Film Induces Morphological Changes and Differentiation of Tendon StemProgenitor Cells. Applied Bionics and Biomechanics،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1120212
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Lu, Kang…[et al.]. Bionic Silk Fibroin Film Induces Morphological Changes and Differentiation of Tendon StemProgenitor Cells. Applied Bionics and Biomechanics No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1120212
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Lu, Kang& Chen, Xiaodie& Tang, Hong& Zhou, Mei& He, Gang& Liu, Juan…[et al.]. Bionic Silk Fibroin Film Induces Morphological Changes and Differentiation of Tendon StemProgenitor Cells. Applied Bionics and Biomechanics. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1120212
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1120212
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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