Bionic Silk Fibroin Film Induces Morphological Changes and Differentiation of Tendon StemProgenitor Cells
Joint Authors
Lai, Fan
Lu, Kang
Chen, Xiaodie
Tang, Hong
Zhou, Mei
He, Gang
Bian, Xuting
Guo, Yupeng
Yang, Mingyu
Lu, Zhisong
Tang, Kanglai
Liu, Juan
Source
Applied Bionics and Biomechanics
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-12-02
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Purpose.
Tendon injuries are common musculoskeletal system disorders, but the ability for tendon regeneration is limited.
Silk fibroin (SF) film may be suitable for tendon regeneration due to its excellent biocompatibility and physical properties.
This study is aimed at evaluating the application value of bionic SF film in tendon regeneration.
Methods.
Tendon stem/progenitor cells (TSPCs) were isolated from rat Achilles tendon and characterized based on their surface marker expression and multilineage differentiation potential.
SF films with smooth or bionic microstructure surfaces (5, 10, 15, 20 μm) were prepared.
The morphology and mechanical properties of natural tendons and SF films were characterized.
TSPCs were used as the seed cells, and the cell viability and cell adhesion morphology were analyzed.
The tendongenesis-related gene expression of TSPCs was also evaluated using quantitative polymerase chain reaction.
Results.
Compared to the native tendon, only the 10, 15, and 20 μm SF film groups had comparable maximum loading and ultimate stress, with the exception of the breaking elongation rate.
The 10 μm SF film group had the highest percentage of oriented cells and the most significant changes in cell morphology.
The most significant upregulations in the expression of COL1A1, TNC, TNMD, and SCX were also observed in the 10 μm SF film group.
Conclusion.
SF film with a bionic microstructure can serve as a tissue engineering scaffold and provide biophysical cues for the use of TSPCs to achieve proper cellular adherence arrangement and morphology as well as promote the tenogenic differentiation of TSPCs, making it a valuable customizable biomaterial for future applications in tendon repair.
American Psychological Association (APA)
Lu, Kang& Chen, Xiaodie& Tang, Hong& Zhou, Mei& He, Gang& Liu, Juan…[et al.]. 2020. Bionic Silk Fibroin Film Induces Morphological Changes and Differentiation of Tendon StemProgenitor Cells. Applied Bionics and Biomechanics،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1120212
Modern Language Association (MLA)
Lu, Kang…[et al.]. Bionic Silk Fibroin Film Induces Morphological Changes and Differentiation of Tendon StemProgenitor Cells. Applied Bionics and Biomechanics No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1120212
American Medical Association (AMA)
Lu, Kang& Chen, Xiaodie& Tang, Hong& Zhou, Mei& He, Gang& Liu, Juan…[et al.]. Bionic Silk Fibroin Film Induces Morphological Changes and Differentiation of Tendon StemProgenitor Cells. Applied Bionics and Biomechanics. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1120212
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1120212