A Sensitive and Rapid Method for Detecting Formaldehyde in Brain Tissues

المؤلفون المشاركون

Yue, Xiangpei
Zhang, Yaoyue
Xing, Wen
Chen, Yutong
Mu, Chenyang
Miao, Zhan
Ge, Peichun
Li, Tingting
Tong, Zhiqian
He, Rong-Qiao

المصدر

Analytical Cellular Pathology

العدد

المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-8، 8ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2017-09-24

دولة النشر

مصر

عدد الصفحات

8

التخصصات الرئيسية

الأمراض
الطب البشري

الملخص EN

The existing methods for detecting formaldehyde (FA) in brain samples are expensive and require sophisticated experimental procedures.

Here, we established a highly sensitive and selective spectrophotometric method, which is based on a reaction in which FA reacts with colorless reagent 4-amino-3-penten-2-one (Fluoral-P) to produce a yellow compound, 3,5-diacetyl-1,4-dihydrolutidine (DDL), which can be detected by a spectrophotometer at 420 nm at room temperature.

The sensitive response time point was found to be at the first hour, and the optimal pH of derivative reaction was pH 6.0.

The limit of detection (LOD) and the limits of quantization (LOQ) for detecting FA were 0.5 μM and 2.5 μM, respectively.

Using this method, an abnormally high level of FA was detected in both the brains of FA-injected mice and autopsy hippocampus tissues from patients with Alzheimer’s disease.

This finding suggests that the modified Fluoral-P method is effective for measuring levels of FA in the brains.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Yue, Xiangpei& Zhang, Yaoyue& Xing, Wen& Chen, Yutong& Mu, Chenyang& Miao, Zhan…[et al.]. 2017. A Sensitive and Rapid Method for Detecting Formaldehyde in Brain Tissues. Analytical Cellular Pathology،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1121737

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Yue, Xiangpei…[et al.]. A Sensitive and Rapid Method for Detecting Formaldehyde in Brain Tissues. Analytical Cellular Pathology No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1121737

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Yue, Xiangpei& Zhang, Yaoyue& Xing, Wen& Chen, Yutong& Mu, Chenyang& Miao, Zhan…[et al.]. A Sensitive and Rapid Method for Detecting Formaldehyde in Brain Tissues. Analytical Cellular Pathology. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1121737

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1121737