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A Sensitive and Rapid Method for Detecting Formaldehyde in Brain Tissues
Joint Authors
Yue, Xiangpei
Zhang, Yaoyue
Xing, Wen
Chen, Yutong
Mu, Chenyang
Miao, Zhan
Ge, Peichun
Li, Tingting
Tong, Zhiqian
He, Rong-Qiao
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-09-24
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The existing methods for detecting formaldehyde (FA) in brain samples are expensive and require sophisticated experimental procedures.
Here, we established a highly sensitive and selective spectrophotometric method, which is based on a reaction in which FA reacts with colorless reagent 4-amino-3-penten-2-one (Fluoral-P) to produce a yellow compound, 3,5-diacetyl-1,4-dihydrolutidine (DDL), which can be detected by a spectrophotometer at 420 nm at room temperature.
The sensitive response time point was found to be at the first hour, and the optimal pH of derivative reaction was pH 6.0.
The limit of detection (LOD) and the limits of quantization (LOQ) for detecting FA were 0.5 μM and 2.5 μM, respectively.
Using this method, an abnormally high level of FA was detected in both the brains of FA-injected mice and autopsy hippocampus tissues from patients with Alzheimer’s disease.
This finding suggests that the modified Fluoral-P method is effective for measuring levels of FA in the brains.
American Psychological Association (APA)
Yue, Xiangpei& Zhang, Yaoyue& Xing, Wen& Chen, Yutong& Mu, Chenyang& Miao, Zhan…[et al.]. 2017. A Sensitive and Rapid Method for Detecting Formaldehyde in Brain Tissues. Analytical Cellular Pathology،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1121737
Modern Language Association (MLA)
Yue, Xiangpei…[et al.]. A Sensitive and Rapid Method for Detecting Formaldehyde in Brain Tissues. Analytical Cellular Pathology No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1121737
American Medical Association (AMA)
Yue, Xiangpei& Zhang, Yaoyue& Xing, Wen& Chen, Yutong& Mu, Chenyang& Miao, Zhan…[et al.]. A Sensitive and Rapid Method for Detecting Formaldehyde in Brain Tissues. Analytical Cellular Pathology. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1121737
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1121737