Methylation Changes of Primary Tumors, Monolayer, and Spheroid Tissue Culture Environments in Malignant Melanoma and Breast Carcinoma
المؤلفون المشاركون
Şükrüoğlu Erdoğan, Özge
Kılıç Erciyas, Seda
Bilir, Ayhan
Buğra Tunçer, Şeref
Akdeniz Ödemiş, Demet
Kurul, Sıdıka
Karanlık, Hasan
Cabıoğlu, Neslihan
Yazıcı, Hülya
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-01-17
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Epigenetic changes have major role in the normal development and programming of gene expression.
Aberrant methylation results in carcinogenesis.
The primary objective of our study is to determine whether primary tumor tissue and cultured tumor cells in 2D and 3D tissue culture systems have the same methylation signature for PAX5, TMPRSS2, and SBDS.
These findings will play an important role in developing in vitro model system to understand the effect of methylation inhibitors on primary tumor tissue.
In a previous study PAX5, TMPRSS2, and SBDS genes that we are investigating were reported to be methylated more than 60% in breast cancer and malignant melanoma cell lines.
However, these genes have never been studied in primary tumor tissues.
Thus, primary tumor tissues of breast cancer and malignant melanoma were first grown in 2D and 3D cultures.
Then these two types of tumor tissues and their 2D and 3D cultures were investigated for changes considering methylation levels in PAX5, TMPRSS2, and SBDS genes using real-time polymerase chain reaction.
No differences were observed in the primary tissues and culture systems for both PAX5 and TMPRSS2 in malignant melanoma tissues.
We found that PAX5 gene was an efficient marker to measure the effects of methylation inhibitors for in vitro systems for malignant melanoma tissue.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Şükrüoğlu Erdoğan, Özge& Kılıç Erciyas, Seda& Bilir, Ayhan& Buğra Tunçer, Şeref& Akdeniz Ödemiş, Demet& Kurul, Sıdıka…[et al.]. 2019. Methylation Changes of Primary Tumors, Monolayer, and Spheroid Tissue Culture Environments in Malignant Melanoma and Breast Carcinoma. BioMed Research International،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1123329
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Şükrüoğlu Erdoğan, Özge…[et al.]. Methylation Changes of Primary Tumors, Monolayer, and Spheroid Tissue Culture Environments in Malignant Melanoma and Breast Carcinoma. BioMed Research International No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1123329
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Şükrüoğlu Erdoğan, Özge& Kılıç Erciyas, Seda& Bilir, Ayhan& Buğra Tunçer, Şeref& Akdeniz Ödemiş, Demet& Kurul, Sıdıka…[et al.]. Methylation Changes of Primary Tumors, Monolayer, and Spheroid Tissue Culture Environments in Malignant Melanoma and Breast Carcinoma. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1123329
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1123329
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر