Methylation Changes of Primary Tumors, Monolayer, and Spheroid Tissue Culture Environments in Malignant Melanoma and Breast Carcinoma
Joint Authors
Şükrüoğlu Erdoğan, Özge
Kılıç Erciyas, Seda
Bilir, Ayhan
Buğra Tunçer, Şeref
Akdeniz Ödemiş, Demet
Kurul, Sıdıka
Karanlık, Hasan
Cabıoğlu, Neslihan
Yazıcı, Hülya
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-01-17
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Epigenetic changes have major role in the normal development and programming of gene expression.
Aberrant methylation results in carcinogenesis.
The primary objective of our study is to determine whether primary tumor tissue and cultured tumor cells in 2D and 3D tissue culture systems have the same methylation signature for PAX5, TMPRSS2, and SBDS.
These findings will play an important role in developing in vitro model system to understand the effect of methylation inhibitors on primary tumor tissue.
In a previous study PAX5, TMPRSS2, and SBDS genes that we are investigating were reported to be methylated more than 60% in breast cancer and malignant melanoma cell lines.
However, these genes have never been studied in primary tumor tissues.
Thus, primary tumor tissues of breast cancer and malignant melanoma were first grown in 2D and 3D cultures.
Then these two types of tumor tissues and their 2D and 3D cultures were investigated for changes considering methylation levels in PAX5, TMPRSS2, and SBDS genes using real-time polymerase chain reaction.
No differences were observed in the primary tissues and culture systems for both PAX5 and TMPRSS2 in malignant melanoma tissues.
We found that PAX5 gene was an efficient marker to measure the effects of methylation inhibitors for in vitro systems for malignant melanoma tissue.
American Psychological Association (APA)
Şükrüoğlu Erdoğan, Özge& Kılıç Erciyas, Seda& Bilir, Ayhan& Buğra Tunçer, Şeref& Akdeniz Ödemiş, Demet& Kurul, Sıdıka…[et al.]. 2019. Methylation Changes of Primary Tumors, Monolayer, and Spheroid Tissue Culture Environments in Malignant Melanoma and Breast Carcinoma. BioMed Research International،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1123329
Modern Language Association (MLA)
Şükrüoğlu Erdoğan, Özge…[et al.]. Methylation Changes of Primary Tumors, Monolayer, and Spheroid Tissue Culture Environments in Malignant Melanoma and Breast Carcinoma. BioMed Research International No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1123329
American Medical Association (AMA)
Şükrüoğlu Erdoğan, Özge& Kılıç Erciyas, Seda& Bilir, Ayhan& Buğra Tunçer, Şeref& Akdeniz Ödemiş, Demet& Kurul, Sıdıka…[et al.]. Methylation Changes of Primary Tumors, Monolayer, and Spheroid Tissue Culture Environments in Malignant Melanoma and Breast Carcinoma. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1123329
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1123329