Transcriptome Analysis Reveals the Molecular Mechanisms Underlying Adenosine Biosynthesis in Anamorph Strain of Caterpillar Fungus

المؤلفون المشاركون

Lin, Shan
Zou, Zhicheng
Zhou, Cuibing
Zhang, Hancheng
Cai, Zhiming

المصدر

BioMed Research International

العدد

المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-12، 12ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2019-12-11

دولة النشر

مصر

عدد الصفحات

12

التخصصات الرئيسية

الطب البشري

الملخص EN

Caterpillar fungus is a well-known fungal Chinese medicine.

To reveal molecular changes during early and late stages of adenosine biosynthesis, transcriptome analysis was performed with the anamorph strain of caterpillar fungus.

A total of 2,764 differentially expressed genes (DEGs) were identified ( p≤0.05, |log2 Ratio| ≥ 1), of which 1,737 were up-regulated and 1,027 were down-regulated.

Gene expression profiling on 4–10 d revealed a distinct shift in expression of the purine metabolism pathway.

Differential expression of 17 selected DEGs which involved in purine metabolism (map00230) were validated by qPCR, and the expression trends were consistent with the RNA-Seq results.

Subsequently, the predicted adenosine biosynthesis pathway combined with qPCR and gene expression data of RNA-Seq indicated that the increased adenosine accumulation is a result of down-regulation of ndk, ADK, and APRT genes combined with up-regulation of AK gene.

This study will be valuable for understanding the molecular mechanisms of the adenosine biosynthesis in caterpillar fungus.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Lin, Shan& Zou, Zhicheng& Zhou, Cuibing& Zhang, Hancheng& Cai, Zhiming. 2019. Transcriptome Analysis Reveals the Molecular Mechanisms Underlying Adenosine Biosynthesis in Anamorph Strain of Caterpillar Fungus. BioMed Research International،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1123551

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Lin, Shan…[et al.]. Transcriptome Analysis Reveals the Molecular Mechanisms Underlying Adenosine Biosynthesis in Anamorph Strain of Caterpillar Fungus. BioMed Research International No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1123551

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Lin, Shan& Zou, Zhicheng& Zhou, Cuibing& Zhang, Hancheng& Cai, Zhiming. Transcriptome Analysis Reveals the Molecular Mechanisms Underlying Adenosine Biosynthesis in Anamorph Strain of Caterpillar Fungus. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1123551

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1123551