Transcriptome Analysis Reveals the Molecular Mechanisms Underlying Adenosine Biosynthesis in Anamorph Strain of Caterpillar Fungus
Joint Authors
Lin, Shan
Zou, Zhicheng
Zhou, Cuibing
Zhang, Hancheng
Cai, Zhiming
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-12-11
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Caterpillar fungus is a well-known fungal Chinese medicine.
To reveal molecular changes during early and late stages of adenosine biosynthesis, transcriptome analysis was performed with the anamorph strain of caterpillar fungus.
A total of 2,764 differentially expressed genes (DEGs) were identified ( p≤0.05, |log2 Ratio| ≥ 1), of which 1,737 were up-regulated and 1,027 were down-regulated.
Gene expression profiling on 4–10 d revealed a distinct shift in expression of the purine metabolism pathway.
Differential expression of 17 selected DEGs which involved in purine metabolism (map00230) were validated by qPCR, and the expression trends were consistent with the RNA-Seq results.
Subsequently, the predicted adenosine biosynthesis pathway combined with qPCR and gene expression data of RNA-Seq indicated that the increased adenosine accumulation is a result of down-regulation of ndk, ADK, and APRT genes combined with up-regulation of AK gene.
This study will be valuable for understanding the molecular mechanisms of the adenosine biosynthesis in caterpillar fungus.
American Psychological Association (APA)
Lin, Shan& Zou, Zhicheng& Zhou, Cuibing& Zhang, Hancheng& Cai, Zhiming. 2019. Transcriptome Analysis Reveals the Molecular Mechanisms Underlying Adenosine Biosynthesis in Anamorph Strain of Caterpillar Fungus. BioMed Research International،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1123551
Modern Language Association (MLA)
Lin, Shan…[et al.]. Transcriptome Analysis Reveals the Molecular Mechanisms Underlying Adenosine Biosynthesis in Anamorph Strain of Caterpillar Fungus. BioMed Research International No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1123551
American Medical Association (AMA)
Lin, Shan& Zou, Zhicheng& Zhou, Cuibing& Zhang, Hancheng& Cai, Zhiming. Transcriptome Analysis Reveals the Molecular Mechanisms Underlying Adenosine Biosynthesis in Anamorph Strain of Caterpillar Fungus. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1123551
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1123551