Applicability of FTIR-ATR Method to Measure Carbonyls in Blood Plasma after Physical and Mental Stress
المؤلفون المشاركون
Komorowska, Małgorzata
Bujok, Jolanta
Gąsior-Głogowska, Marlena
Marszałek, Michał
Trochanowska-Pauk, Natalia
Zigo, František
Pavľak, Alexander
Walski, Tomasz
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-03-26
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Introduction.
Oxidative stress is a state of imbalance between the production of reactive oxygen species and antioxidant defenses.
It results in the oxidation of all cellular elements and, to a large extent, proteins, causing inter alia the formation of carbonyl groups in their structures.
The study focused on assessment of changes in the plasma protein-bound carbonyls in police horses after combat training and after rest and the applicability of infrared spectroscopy with a Fourier transform, utilizing the attenuated total reflectance (FTIR-ATR) in detecting plasma protein oxidation.
Methods.
We evaluated the influence of both the different concentrations of hydrogen peroxide and combat training on protein carbonylation in horse blood plasma.
The oxidation of plasma proteins was assessed using a spectrophotometric method based on the carbonyl groups derivatization with 2,4-dinitrophenylhydrazine (DNPH).
The measured values were correlated with the carbonyl groups concentrations determined by means of the FTIR-ATR method.
Results.
The linear correlation between the DNPH and FTIR-ATR methods was shown.
The concentration of plasma protein-bound carbonyls significantly deceased in police horses after one-day rest when compared to the values measured directly after the combat training (a drop by 23%, p<0.05 and 29%, p<0.01 measured by DNPH and FTIR-ATR methods, respectively).
These results were consistent with the proteins phosphorylation analysis.
Conclusion.
The FTIR-ATR method may be applied to measure the level of plasma proteins peroxidation.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Bujok, Jolanta& Gąsior-Głogowska, Marlena& Marszałek, Michał& Trochanowska-Pauk, Natalia& Zigo, František& Pavľak, Alexander…[et al.]. 2019. Applicability of FTIR-ATR Method to Measure Carbonyls in Blood Plasma after Physical and Mental Stress. BioMed Research International،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1123738
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Bujok, Jolanta…[et al.]. Applicability of FTIR-ATR Method to Measure Carbonyls in Blood Plasma after Physical and Mental Stress. BioMed Research International No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1123738
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Bujok, Jolanta& Gąsior-Głogowska, Marlena& Marszałek, Michał& Trochanowska-Pauk, Natalia& Zigo, František& Pavľak, Alexander…[et al.]. Applicability of FTIR-ATR Method to Measure Carbonyls in Blood Plasma after Physical and Mental Stress. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1123738
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1123738
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر