Applicability of FTIR-ATR Method to Measure Carbonyls in Blood Plasma after Physical and Mental Stress
Joint Authors
Komorowska, Małgorzata
Bujok, Jolanta
Gąsior-Głogowska, Marlena
Marszałek, Michał
Trochanowska-Pauk, Natalia
Zigo, František
Pavľak, Alexander
Walski, Tomasz
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-03-26
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Introduction.
Oxidative stress is a state of imbalance between the production of reactive oxygen species and antioxidant defenses.
It results in the oxidation of all cellular elements and, to a large extent, proteins, causing inter alia the formation of carbonyl groups in their structures.
The study focused on assessment of changes in the plasma protein-bound carbonyls in police horses after combat training and after rest and the applicability of infrared spectroscopy with a Fourier transform, utilizing the attenuated total reflectance (FTIR-ATR) in detecting plasma protein oxidation.
Methods.
We evaluated the influence of both the different concentrations of hydrogen peroxide and combat training on protein carbonylation in horse blood plasma.
The oxidation of plasma proteins was assessed using a spectrophotometric method based on the carbonyl groups derivatization with 2,4-dinitrophenylhydrazine (DNPH).
The measured values were correlated with the carbonyl groups concentrations determined by means of the FTIR-ATR method.
Results.
The linear correlation between the DNPH and FTIR-ATR methods was shown.
The concentration of plasma protein-bound carbonyls significantly deceased in police horses after one-day rest when compared to the values measured directly after the combat training (a drop by 23%, p<0.05 and 29%, p<0.01 measured by DNPH and FTIR-ATR methods, respectively).
These results were consistent with the proteins phosphorylation analysis.
Conclusion.
The FTIR-ATR method may be applied to measure the level of plasma proteins peroxidation.
American Psychological Association (APA)
Bujok, Jolanta& Gąsior-Głogowska, Marlena& Marszałek, Michał& Trochanowska-Pauk, Natalia& Zigo, František& Pavľak, Alexander…[et al.]. 2019. Applicability of FTIR-ATR Method to Measure Carbonyls in Blood Plasma after Physical and Mental Stress. BioMed Research International،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1123738
Modern Language Association (MLA)
Bujok, Jolanta…[et al.]. Applicability of FTIR-ATR Method to Measure Carbonyls in Blood Plasma after Physical and Mental Stress. BioMed Research International No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1123738
American Medical Association (AMA)
Bujok, Jolanta& Gąsior-Głogowska, Marlena& Marszałek, Michał& Trochanowska-Pauk, Natalia& Zigo, František& Pavľak, Alexander…[et al.]. Applicability of FTIR-ATR Method to Measure Carbonyls in Blood Plasma after Physical and Mental Stress. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1123738
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1123738