Quantitative Proteomic Analysis of Human Seminal Plasma from Normozoospermic and Asthenozoospermic Individuals
المؤلفون المشاركون
Wu, Yibo
Yuan, Yan
Chen, Ling
Wang, Min
Yang, Yong
Wang, Yingnan
Quan, Chao
Chen, Daozhen
Chen, Ying
Huang, Xiaoyan
Zhou, Tao
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-03-10
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Seminal plasma is a complex mixture of secretions from various glands in the male genital tract.
Compared to sperm cells, it contains important proteins that are both directly and indirectly associated with sperm motility.
Here, we constructed quantitative proteomes of human seminal plasma from three normozoospermic and asthenozoospermic individuals.
A total of 524 proteins were identified, and 366 of them were found to be quantified in all six samples.
We first investigated the absolute expression features of these proteins and found that the variations of protein identification among different samples and other published datasets were mainly due to some lowly expressed proteins.
By integration of various proteomic datasets and bioinformatics databases, we comprehensively annotated the biological functions, physiological originations, and disease associations of these proteins.
We found that our dataset could benefit the studies of both male infertility and other male diseases.
Finally, based on the relative expression values determined by chemical labeling, we identified a total of 29 differentially expressed proteins, which could be used as candidate targets for studying the molecular bases of sperm motility or developing precise diagnostic biomarkers of asthenozoospermia.
We further successfully verified the expression trends of four representative proteins by Western blotting.
Compared to a previous dataset based on label-free quantification, our results showed that most of the important proteins could be identified in the sample collected only once for each individual, providing the bases for personalized examination of seminal plasma proteins in clinic.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wu, Yibo& Yuan, Yan& Chen, Ling& Wang, Min& Yang, Yong& Wang, Yingnan…[et al.]. 2019. Quantitative Proteomic Analysis of Human Seminal Plasma from Normozoospermic and Asthenozoospermic Individuals. BioMed Research International،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1124049
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wu, Yibo…[et al.]. Quantitative Proteomic Analysis of Human Seminal Plasma from Normozoospermic and Asthenozoospermic Individuals. BioMed Research International No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1124049
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wu, Yibo& Yuan, Yan& Chen, Ling& Wang, Min& Yang, Yong& Wang, Yingnan…[et al.]. Quantitative Proteomic Analysis of Human Seminal Plasma from Normozoospermic and Asthenozoospermic Individuals. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1124049
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1124049
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر